Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice
In vitro studies of pure tubulin have suggested that tubulin heterodimers in cells assemble into B-lattice microtubules, where the 8-nm dimers in adjacent protofilaments are staggered by 0.9 nm. This arrangement requires the tube to close by forming a seam with an A-lattice, in which the protofilame...
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Published in | Nature structural & molecular biology Vol. 15; no. 10; pp. 1102 - 1108 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Nature Publishing Group
01.10.2008
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Subjects | |
Online Access | Get full text |
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Summary: | In vitro studies of pure tubulin have suggested that tubulin heterodimers in cells assemble into B-lattice microtubules, where the 8-nm dimers in adjacent protofilaments are staggered by 0.9 nm. This arrangement requires the tube to close by forming a seam with an A-lattice, in which the protofilaments are staggered by 4.9 nm. Here we show that Mal3, an EB1 family tip-tracking protein, drives tubulin to assemble in vitro into exclusively 13-protofilament microtubules with a high proportion of A-lattice protofilament contacts. We present a three-dimensional cryo-EM reconstruction of a purely A-lattice microtubule decorated with Mal3, in which Mal3 occupies the groove between protofilaments and associates closely with one tubulin monomer. We propose that Mal3 promotes assembly by binding to freshly formed tubulin polymer and particularly favors any with A-lattice arrangement. These results reopen the question of microtubule structure in cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 A.d.G., M.K., D.R.D., R.A.C. and L.A.A. designed the experiments and wrote the manuscript; A.d.G. and M.K. performed the experiments; D.R.D. made strain mmsp174 and plasmid Mal-308; A.d.G. & L.A.A. performed the image analysis; M.O. prepared the S. pombe tubulin. AUTHOR CONTRIBUTIONS |
ISSN: | 1545-9993 1545-9985 |
DOI: | 10.1038/nsmb.1482 |