Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profile

• Published in: Cytotechnology (Dordrecht) Vol. 63; no. 1; pp. 57 - 66
• Main Authors: Jung, Hana, Kim, Hak Hee, Lee, Dong Hee, Hwang, Yu-Shik, Yang, Hyeong-Cheol, Park, Jong-Chul
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 2011; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Adipose derived stem cells (ADSCs); Antibodies; Biochemistry; Biological and medical sciences; Biomedicine; Biotechnology; Body fat; Cell migration; Chemistry; Chemistry and Materials Science; Collagen; Collagen (type I); Collagen (type III); Enzymes; Extracellular matrix; Fibroblasts; Fundamental and applied biological sciences. Psychology; Gene expression; Growth factors; Human dermal fibroblasts (HDFs); Hyaluronic acid; Hyaluronic acid synthase (HAS); mRNA; Original Research; Paracrine effect; Paracrine signalling; Protein expression; Proteins; Secretion; Skin; Statistical analysis; Stem cells; TGF-β1; Transforming growth factor- beta 1; Transforming growth factor-b1; Variance analysis; Wound healing; United States > US; Paracrine effect; Hyaluronic acid synthase (HAS); TGF-β1; Adipose derived stem cells (ADSCs); Human dermal fibroblasts (HDFs); Human; Enzyme; Stem cell; Transforming growth factor; Synthase; Collagen; Hyaluronic acid; Fibroblast
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-010-9327-4

Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). However, the main soluble factor of ADSC-CM which mediates paracrine effects and its underlying mechanism has not been elucidated yet. In this study, we considered transforming growth factor-beta1 (TGF-β1) as a strong candidate for paracrine effect of ADSC-CM and investigated collagen synthesis and hyaluronic acid synthase (HAS) expression. After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. Furthermore, to clarify effects of TGF-β1 as a paracrine mediator, TGF-β1 antibody and external supplementary TGF-β1 were treated to HDFs. Collagens type I, type III, HAS-1 and HAS-2 mRNA expressions of HDFs were greatly increased by ADSC-CM treatment, however there was no change in TGF-β1 antibody treated HDFs compared with non-treated control. These results strongly demonstrate that TGF-β1 plays an important role as a paracrine mediator of ECM synthesis. The fact that TGF-β1 contained in ADSC-CM not only accelerates collagen deposition but also increase hyaluronic acid synthesis of HDFs through HAS-1 and HAS-2 expression was also elucidated in this study. Therefore, ADSC-CM shows promise for the treatment of cutaneous wounds and accelerates granulation formation during healing process.