The Correlation between Zeta Potential and Mucoadhesion Strength on Pig Vesical Mucosa

• Published in: Biological & Pharmaceutical Bulletin Vol. 26; no. 5; pp. 743 - 746
• Main Authors: Bogataj, Marija, Vovk, Tomaz, Kerec, Mojca, Dimnik, Aleš, Grabnar, Iztok, Mrhar, Aleš
• Format: Journal Article
• Language: English; Japanese
• Published: Tokyo The Pharmaceutical Society of Japan 2003; Pharmaceutical Society of Japan; Maruzen; Japan Science and Technology Agency
• Subjects: Acrylic Resins - chemistry; Adhesiveness; Animals; Biological and medical sciences; Carboxymethylcellulose Sodium - chemistry; Chitin - analogs & derivatives; Chitin - chemistry; Chitosan; chitosan hydrochloride; Drug Carriers - chemistry; Female; In Vitro Techniques; Male; Medical sciences; mucoadhesion; polycarbophil; sodium carboxymethyl cellulose; Static Electricity; Swine; Urinary Bladder - chemistry; urinary bladder mucosa; Urothelium - chemistry; zeta potential; zeta potential; mucoadhesion; urinary bladder mucosa; chitosan hydrochloride; polycarbophil; sodium carboxymethyl cellulose
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.26.743

The detachment forces of various polymers are frequently measured to determine their mucoadhesion strength. As the process of mucoadhesion is a consequence of interactions between the mucus layer on mucosa and mucoadhesive polymers, it is greatly dependent on mucus and polymer structure including their charge. It is also known that the glycosaminoglycan layer, which covers the urinary bladder mucosa surface, is highly negatively charged. Therefore, by measuring the zeta potential of polymer dispersions and mucosal homogenates an insight into electrostatic interactions during mucoadhesion can be obtained. In our experiments we chose three polymers, two anionic (polycarbophil, PC; sodium carboxymethyl cellulose, CMCNa) and one cationic (chitosan hydrochloride, CH), for which we expected different zeta potential values and different mucoadhesion strengths. The correlation between the zeta potential and the detachment force was determined. In addition to that, the zeta potential of the scraped surface layer of pig urinary bladders was measured to confirm its negative value. The mucoadhesion strength decreased in the following order: CH>CMCNa=PC. The zeta potentials for all three polymers and for porcine vesical mucosal homogenates were measured in Tyrode solution and two NaCl solutions with different ionic strengths. The lower values of the detachment force correlated well with the more negative zeta potential of the polymer, which might be a consequence of the greater repulsion between negative charges of polymers and glycosaminoglycans.