Utilization of Lactic Acid Bacterial Genes in Synechocystis sp. PCC 6803 in the Production of Lactic Acid

• Published in: Bioscience, biotechnology, and biochemistry Vol. 77; no. 5; pp. 966 - 970
• Main Authors: JOSEPH, Ancy, AIKAWA, Shimpei, SASAKI, Kengo, TSUGE, Yota, MATSUDA, Fumio, TANAKA, Tsutomu, KONDO, Akihiko
• Format: Journal Article
• Language: English
• Published: England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013; Oxford University Press
• Subjects: Bacteria; biochemical pathways; biotechnology; carbon dioxide; Cell Membrane - secretion; Cyanobacteria; genes; genetic engineering; Genetic Engineering - methods; L-Lactate Dehydrogenase - genetics; lactate dehydrogenase; lactic acid; Lactic Acid - biosynthesis; Lactic Acid - chemistry; Lactic Acid - isolation & purification; Lactic Acid - metabolism; lactic acid bacteria; Lactobacillus - enzymology; Lactobacillus - genetics; Lactobacillus - metabolism; Lactobacillus plantarum; Lactobacillus rhamnosus; Lactococcus - enzymology; Lactococcus - genetics; Lactococcus - metabolism; Lactococcus lactis; Monocarboxylic Acid Transporters - genetics; reverse transcriptase polymerase chain reaction; secretion; Stereoisomerism; Synechocystis; Synechocystis - cytology; Synechocystis - genetics; Synechocystis - growth & development; Synechocystis - metabolism; Synechocystis sp; Transformation, Bacterial
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.120921

Metabolic pathway engineering of cyanobacteria for the production of industrially important chemicals from atmospheric CO2 has generated interest recently. Here, we engineered Synechocystis sp. PCC 6803 to produce lactic acid using a lactate dehydrogenase (ldh) gene from various lactic acid-producing bacteria, Lactococcus lactis (ldhB and ldhX), Lactobacillus plantarum (ldhL and ldh), and Lactobacillus rhamnosus (ldhL). The lactic acid was secreted outside the cell using a transporter (lldp) gene from L. plantarum. Expression of each ldh in Synechocystis sp. PCC6803 was ascertained by reverse-transcriptase polymerase chain reaction. Five transformants led to the production of L-lactic acid. Co-expression of lldp with ldhB from L. plantarum or ldhL from L. rhamnosus led to the secretion of lactic acid into the medium at concentration of 0.17 ± 0.02 or 0.14 ± 0.02 mM after 18 d of cultivation.