Protective effect of the methanol extract of edible insects against oxidative damages in C2C12 myoblasts and myotubes

This study investigated the protective effect of edible insect methanol extracts (EIMEs) against oxidative stress induced by H2O2 in C2C12 cells. Total phenolic and total flavonoid contents of EIMEs were 7.43–47.15 mg gallic acid equivalents/g of residue and 2.01–31.00 mg catechin equivalents/g of r...

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Published inFOOD SCIENCE AND TECHNOLOGY RESEARCH Vol. 29; no. 4; pp. 339 - 346
Main Authors Kim, Minha, Lee, Junsoo, Jeong, Taekyoung, Heo, Huijin, Park, Huirim, Lee, Hana
Format Journal Article
LanguageEnglish
Published Tsukuba Japanese Society for Food Science and Technology 01.01.2023
The Japanese Society for Food Science and Technology
Japan Science and Technology Agency
Subjects
C2C12 cells
Catechin
Creatine
Creatine kinase
Cytotoxicity
Damage
edible insects
Equivalence
Flavonoids
Food sources
Gallic acid
Gene expression
Glutathione
Hydrogen peroxide
Insects
Kinases
L-Lactate dehydrogenase
Lactate dehydrogenase
Lipid peroxidation
Lipids
Methanol
mRNA
Muscles
Myoblasts
Myosin
Myotubes
Oxidative stress
Peroxidation
Phenolic compounds
Phenols
protective effect
Reactive oxygen species
Residues
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Summary:This study investigated the protective effect of edible insect methanol extracts (EIMEs) against oxidative stress induced by H2O2 in C2C12 cells. Total phenolic and total flavonoid contents of EIMEs were 7.43–47.15 mg gallic acid equivalents/g of residue and 2.01–31.00 mg catechin equivalents/g of residue, respectively. Among edible insects, Oxya chinensis showed the highest antioxidant activity, followed by Allomyrina dichotoma. EIMEs (100 µg/mL) significantly prevented H2O2-induced cytotoxicity in both myoblasts and myotubes. H2O2-induced oxidative stress causes reactive oxygen species (ROS) production, glutathione depletion, and lipid peroxidation, all of which were markedly ameliorated by EIMEs in myoblasts in our study. In addition, A. dichotoma prevented the generation of ROS and release of creatine kinase (CK) and lactate dehydrogenase (LDH) in myotubes. EIMEs increased the diameter of myotubes and mRNA and protein expression levels of myosin-heavy chains. Our study provides insights into the application of edible insects to regulate muscle differentiation and alleviate oxidative damage to muscles.
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ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.FSTR-D-23-00007