Characterization of the testicular regeneration potential in premature cockerels

Previous studies have shown that grafted neonatal chicken testicular tissue can develop and produce functional sperm; however, it was unclear whether regenerative processes or proportional growth caused the re-appearance of spermatogenic tissue. We dissociated testicular tissues, performed subcutane...

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Published inJournal of Reproduction and Development Vol. 63; no. 6; pp. 563 - 570
Main Authors CHENG, Po-Liang, WU, Hui-Ru, LI, Cheng-Yan, CHEN, Chih-Feng, CHENG, Hsu-Chen
Format Journal Article
LanguageEnglish
Published Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 2017
Japan Science and Technology Agency
The Society for Reproduction and Development
Subjects
Auto-transplantation
Cockerel
Neonates
Original
Regeneration potential
Seminiferous tubule
Sperm
Spermatogenesis
Surgery
Testes
Testis
Testosterone
Transplantation
Testis
Regeneration potential
Auto-transplantation
Spermatogenesis
Cockerel
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Summary:Previous studies have shown that grafted neonatal chicken testicular tissue can develop and produce functional sperm; however, it was unclear whether regenerative processes or proportional growth caused the re-appearance of spermatogenic tissue. We dissociated testicular tissues, performed subcutaneous auto-transplantation of the re-aggregated cells to castrated cockerels, and monitored the post-surgery development of these transplanted aggregates. We found that these transplanted cell aggregates experienced compensatory growth in the form of a 300-fold increase in size, rather than the 30-fold increase observed in normal testis development. Further, these dissociated testicular cell aggregates restored seminiferous tubule structure and were able to produce testosterone and motile sperm. Therefore, we concluded that the dissociated testicular cells from 11-week-old cockerels retained a strong regenerative potential, as they exhibited compensatory growth, restored destroyed structure, and sustained spermatogenesis.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.2017-090