Administration of anti-glucagon-like peptide-2 serum suppresses epithelial cell proliferation of the distal small intestine in weanling rats

• Published in: Biomedical Research Vol. 30; no. 4; pp. 259 - 261
• Main Authors: Ishizuka, Satoshi, Inafune, Ayako, Hira, Tohru, Izumi, Hirohisa, Ozawa, Kazuhiro, Takase, Mitsunori, Hara, Hiroshi
• Format: Journal Article
• Language: English
• Published: Japan Biomedical Research Press 2009; Japan Science and Technology Agency
• Subjects: Animals; Cell Proliferation - drug effects; Glucagon-Like Peptide 2 - antagonists & inhibitors; Glucagon-Like Peptide 2 - immunology; Glucagon-Like Peptide 2 - metabolism; Ileum - cytology; Ileum - growth & development; Ileum - immunology; Ileum - metabolism; Immune Sera - immunology; Immune Sera - pharmacology; Intestinal Mucosa - cytology; Intestinal Mucosa - growth & development; Intestinal Mucosa - immunology; Intestinal Mucosa - metabolism; Male; Rats; Rats, Sprague-Dawley; Weaning
• ISSN: 0388-6107; 1880-313X
• DOI: 10.2220/biomedres.30.259

We investigated the effects of endogenous glucagon-like peptide-2 (GLP-2) on the development of intestinal mucosa in weanling rats. Three-week-old male weanling Sprague-Dawley rats were administered either anti-GLP-2 or normal rabbit serum every other day for 2 weeks. We then measured length, weight, and bromodeoxyuridine incorporation in the intestine on day 13 following the first injection. Administration of anti-GLP-2 serum significantly inhibited both epithelial proliferation in the distal ileum and elongation of the small intestine. These results suggest that intrinsic GLP-2 contributes to the growth of the small intestine during the weanling period.