Development of versatile non-homologous end joining-based knock-in module for genome editing

• Published in: Scientific reports Vol. 8; no. 1; pp. 593 - 10
• Main Authors: Sawatsubashi, Shun, Joko, Yudai, Fukumoto, Seiji, Matsumoto, Toshio, Sugano, Shigeo S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.01.2018; Nature Publishing Group
• Subjects: 13/44; 45/41; 631/1647/1511; 631/61/338; Cloning; Conserved sequence; CRISPR; Genome editing; Genomes; Homology; Humanities and Social Sciences; Keratinocytes; multidisciplinary; Non-homologous end joining; Nucleotide sequence; Phenotypes; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-18911-9

CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering. An important aspect of genome engineering is efficient knock-in technology. For improved knock-in efficiency, the non-homologous end joining (NHEJ) repair pathway has been used over the homology-dependent repair pathway, but there remains a need to reduce the complexity of the preparation of donor vectors. We developed the v ersat i le NHEJ-based k nock- in module for g enome editing (VIKING). Using the consensus sequence of the time-honored pUC vector to cut donor vectors, any vector with a pUC backbone could be used as the donor vector without customization. Conditions required to minimize random integration rates of the donor vector were also investigated. We attempted to isolate null lines of the VDR gene in human HaCaT keratinocytes using knock-in/knock-out with a selection marker cassette, and found 75% of clones isolated were successfully knocked-in. Although HaCaT cells have hypotetraploid genome composition, the results suggest multiple clones have VDR null phenotypes. VIKING modules enabled highly efficient knock-in of any vectors harboring pUC vectors. Users now can insert various existing vectors into an arbitrary locus in the genome. VIKING will contribute to low-cost genome engineering.