癫持续状态大鼠海马组织中肿瘤坏死因子α和核因子κB的变化及其相关性

目的探讨癫持续状态（SE）大鼠海马组织中肿瘤坏死因子α（TNF-α）和核因子κB（NF-κB）的表达。方法对Wistar大鼠用10%匹罗卡品300 mg/kg腹腔注射（SE组,n=20）,产生SE后60 min终止发作;另设注射等量生理盐水的对照组（n=6）。饲养24 h后取大鼠海马组织,苏木精-伊红染色观察海马CA3区神经元数量,Western blotting测定海马组织NF-κB表达,双抗体夹心酶联免疫法（ELISA）测定海马组织TNF-α表达。采用Pearson相关性分析法分析TNF-α和NF-κB表达的相关性。结果 SE组海马CA3区神经元数量（53.26±2.67）明显小于对照组...

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Published in上海交通大学学报（医学版） Vol. 31; no. 12; pp. 1711 - 1714
Main Author 宋莎莎 耿直 高华
Format Journal Article
LanguageChinese
Published 上海交通大学附属第六人民医院神经内科,上海,200233 2011
Subjects
大鼠
核因子κB
癫持续状态
肿瘤坏死因子α
肿瘤坏死因子α
癫(癎)持续状态
大鼠
核因子κB
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ISSN1674-8115
DOI10.3969/j.issn.1674-8115.2011.12.011

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Summary:目的探讨癫持续状态（SE）大鼠海马组织中肿瘤坏死因子α（TNF-α）和核因子κB（NF-κB）的表达。方法对Wistar大鼠用10%匹罗卡品300 mg/kg腹腔注射（SE组,n=20）,产生SE后60 min终止发作;另设注射等量生理盐水的对照组（n=6）。饲养24 h后取大鼠海马组织,苏木精-伊红染色观察海马CA3区神经元数量,Western blotting测定海马组织NF-κB表达,双抗体夹心酶联免疫法（ELISA）测定海马组织TNF-α表达。采用Pearson相关性分析法分析TNF-α和NF-κB表达的相关性。结果 SE组海马CA3区神经元数量（53.26±2.67）明显小于对照组（103.22±0.83）,差异具有统计学意义（P〈0.001）。SE组大鼠海马组织TNF-α和NF-κB表达水平均显著高于对照组（P〈0.001和P〈0.05）,且两者表达水平呈正相关（r=0.457,P=0.02）。结论 SE后24 h,TNF-α和NF-κB在大鼠海马组织中表达活性增强,且两者表达水平呈正相关。
Bibliography:status epilepticus; tumor necrosis factor-α; nuclear factor-κB; rat
31-1259/R
Objective To investigate the expression of tumor necrosis factor-α（TNF-α） and nuclear factor（NF-κB） in hippocampus of rats with status epilepticus（SE）. MethodsTwenty Wistar rats were intraperitoneally injected with 10% pilocarpine（300 mg/kg）（SE group）,and injection was ceased 60 min after SE onset.Besides,six rats treated with same amount of normal saline were served as control group.Hippocampus tissues of rats were obtained 24 h after treatment,the number of neurons in CA3 area of hippocampus was observed with HE staining,the expression of NF-κB and TNF-α in hippocampus tissues was determined by Western blotting and ELISA respectively.Pearson correlation analysis was adopted to explore the correlation between the expression of TNF-α and that of NF-κB. ResultsThe number of neurons in CA3 area of hippocampus in SE group was much smaller than that in control group（53.26±2.67 vs 103.22±0.83,P0.001）.The expression of TNF-α and NF-κB in hi
ISSN:1674-8115
DOI:10.3969/j.issn.1674-8115.2011.12.011