丹参注射液对高糖诱导的大鼠腹膜间皮细胞的过氧化损伤及E钙黏素和α平滑肌肌动蛋白表达的影响
目的研究丹参注射液对高糖作用下的大鼠腹膜间皮细胞过氧化损伤及E钙黏素和α平滑肌肌动蛋白（α-SMA）表达的影响。方法体外培养大鼠原代腹膜间皮细胞,第3代细胞经无血清培养基同步培养24 h后分为正常对照组、高糖诱导组（25 mg/mL葡萄糖）、甘露醇对照组（25 mg/mL甘露醇）和丹参干预组（15 mg/mL丹参注射液＋25 mg/mL葡萄糖）,测定各组细胞内丙二醛（MDA）和活性氧（ROS）含量及超氧化物歧化酶（SOD）活性;采用RT-PCR技术和Western blotting方法检测各组细胞内E钙黏素和α-SMA的mRNA和蛋白表达。结果与正常对照组比较,高糖诱导组细胞内ROS和MDA含...
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| Published in | 上海交通大学学报（医学版） Vol. 32; no. 6; pp. 741 - 745 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海交通大学医学院附属新华医院肾脏内科,上海,200092
2012
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-8115 |
| DOI | 10.3969/j.issn.1674-8115.2012.06.011 |
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| Abstract | 目的研究丹参注射液对高糖作用下的大鼠腹膜间皮细胞过氧化损伤及E钙黏素和α平滑肌肌动蛋白（α-SMA）表达的影响。方法体外培养大鼠原代腹膜间皮细胞,第3代细胞经无血清培养基同步培养24 h后分为正常对照组、高糖诱导组（25 mg/mL葡萄糖）、甘露醇对照组（25 mg/mL甘露醇）和丹参干预组（15 mg/mL丹参注射液＋25 mg/mL葡萄糖）,测定各组细胞内丙二醛（MDA）和活性氧（ROS）含量及超氧化物歧化酶（SOD）活性;采用RT-PCR技术和Western blotting方法检测各组细胞内E钙黏素和α-SMA的mRNA和蛋白表达。结果与正常对照组比较,高糖诱导组细胞内ROS和MDA含量增高,而SOD活性降低,E钙黏素mRNA和蛋白的表达减少,α-SMA mRNA和蛋白的表达增加,组间比较差异均有统计学意义（P〈0.05）。与高糖诱导组比较,丹参干预组细胞内ROS和MDA含量减少,SOD活性增强,E钙黏素mRNA和蛋白的表达增加,而α-SMA mRNA和蛋白的表达减少,组间比较差异均有统计学意义（P〈0.05）。结论丹参注射液可明显减轻高糖诱导的腹膜间皮细胞的过氧化损伤,其对细胞内E钙黏素和α-SMA表达的调控作用可能与延缓腹膜纤维化作用有关。 |
|---|---|
| AbstractList | R322.492; 目的 研究丹参注射液对高糖作用下的大鼠腹膜间皮细胞过氧化损伤及E钙黏素和α平滑肌肌动蛋白(α-SMA)表达的影响.方法 体外培养大鼠原代腹膜间皮细胞,第3代细胞经无血清培养基同步培养24 h后分为正常对照组、高糖诱导组(25 mg/mL葡萄糖)、甘露醇对照组(25 mg/mL甘露醇)和丹参干预组(15 mg/mL丹参注射液+25 mg/mL葡萄糖),测定各组细胞内丙二醛(MDA)和活性氧(ROS)含量及超氧化物歧化酶(SOD)活性；采用RT-PCR技术和Western blotting方法检测各组细胞内E钙黏素和α-SMA的mRNA和蛋白表达.结果 与正常对照组比较,高糖诱导组细胞内ROS和MDA含量增高,而SOD活性降低,E钙黏素mRNA和蛋白的表达减少,α-SMA mRNA和蛋白的表达增加,组间比较差异均有统计学意义(P＜0.05).与高糖诱导组比较,丹参干预组细胞内ROS和MDA含量减少,SOD活性增强,E钙黏素mRNA和蛋白的表达增加,而α-SMA mRNA和蛋白的表达减少,组间比较差异均有统计学意义(P＜0.05).结论 丹参注射液可明显减轻高糖诱导的腹膜间皮细胞的过氧化损伤,其对细胞内E钙黏素和α-SMA表达的调控作用可能与延缓腹膜纤维化作用有关. 目的研究丹参注射液对高糖作用下的大鼠腹膜间皮细胞过氧化损伤及E钙黏素和α平滑肌肌动蛋白（α-SMA）表达的影响。方法体外培养大鼠原代腹膜间皮细胞,第3代细胞经无血清培养基同步培养24 h后分为正常对照组、高糖诱导组（25 mg/mL葡萄糖）、甘露醇对照组（25 mg/mL甘露醇）和丹参干预组（15 mg/mL丹参注射液＋25 mg/mL葡萄糖）,测定各组细胞内丙二醛（MDA）和活性氧（ROS）含量及超氧化物歧化酶（SOD）活性;采用RT-PCR技术和Western blotting方法检测各组细胞内E钙黏素和α-SMA的mRNA和蛋白表达。结果与正常对照组比较,高糖诱导组细胞内ROS和MDA含量增高,而SOD活性降低,E钙黏素mRNA和蛋白的表达减少,α-SMA mRNA和蛋白的表达增加,组间比较差异均有统计学意义（P〈0.05）。与高糖诱导组比较,丹参干预组细胞内ROS和MDA含量减少,SOD活性增强,E钙黏素mRNA和蛋白的表达增加,而α-SMA mRNA和蛋白的表达减少,组间比较差异均有统计学意义（P〈0.05）。结论丹参注射液可明显减轻高糖诱导的腹膜间皮细胞的过氧化损伤,其对细胞内E钙黏素和α-SMA表达的调控作用可能与延缓腹膜纤维化作用有关。 |
| Author | 刘怡晟 朱淳 蒋更如 孙静姝 刘秀英 |
| AuthorAffiliation | 上海交通大学医学院附属新华医院肾脏内科,上海200092 |
| AuthorAffiliation_xml | – name: 上海交通大学医学院附属新华医院肾脏内科,上海,200092 |
| Author_FL | LIU Xiu-ying SUN Jing-shu LIU Yi-sheng ZHU Chun JIANG Geng-ru |
| Author_FL_xml | – sequence: 1 fullname: LIU Yi-sheng – sequence: 2 fullname: ZHU Chun – sequence: 3 fullname: JIANG Geng-ru – sequence: 4 fullname: SUN Jing-shu – sequence: 5 fullname: LIU Xiu-ying |
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| DocumentTitleAlternate | Effects of Salvia Miltiorrhiza injection on expression of E-cadherin/α-smooth muscle actin and peroxidative injury of rat peritoneal mesothelial cells induced by high glucose |
| DocumentTitle_FL | Effects of Salvia Miltiorrhiza injection on expression of E-cadherin/α-smooth muscle actin and peroxidative injury of rat peritoneal mesothelial cells induced by high glucose |
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| Keywords | 丹参 过氧化损伤 α平滑肌肌动蛋白 E钙黏素 腹膜间皮细胞 |
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| Notes | Salvia Miltiorrhiza； peritoneal mesothelial cell； peroxidative injury； E-cadherin； α-smooth muscle actin 31-1259/R LIU Yi-sheng,ZHU Chun,JIANG Geng-ru,SUN Jing-shu,LIU Xiu-ying（Department of Nephrology,Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200092,China） Objective To investigate the effects of Salvia Miltiorrhiza injection on expression of E-cadherin/α-smooth muscle actin（α-SMA） and peroxidative injury of rat peritoneal mesothelial cells induced by high glucose.Methods Rat primary peritoneal mesothelial cells were cultured in vitro.After synchronization for 24 h,primary peritoneal mesothelial cells of the third passage were divided into normal control group,high glucose group（treated with 25 mg/mL glucose）,mannitol control group（treated with 25 mg/mL mannitol） and Salvia Miltiorrhiza group（treated with 15 mg/mL Salvia Miltiorrhiza＋25 mg/mL glucose）.The contents of malondialdehyde（MDA） and reactive oxygen species（ROS） and activity of superoxide dismutase（SOD） in cells were determ |
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| PublicationTitle | 上海交通大学学报（医学版） |
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| PublicationYear | 2012 |
| Publisher | 上海交通大学医学院附属新华医院肾脏内科,上海,200092 |
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| Snippet | 目的研究丹参注射液对高糖作用下的大鼠腹膜间皮细胞过氧化损伤及E钙黏素和α平滑肌肌动蛋白（α-SMA）表达的影响。方法体外培养大鼠原代腹膜间皮细胞,第3代细胞经无血清培养基同步培养24 h后分为正常对照组、高糖诱导组（25 mg/mL葡萄糖）、甘露醇对照组（25 mg/mL甘露醇）和丹参干预组（15... R322.492; 目的 研究丹参注射液对高糖作用下的大鼠腹膜间皮细胞过氧化损伤及E钙黏素和α平滑肌肌动蛋白(α-SMA)表达的影响.方法 体外培养大鼠原代腹膜间皮细胞,第3代细胞经无血清培养基同步培养24 h后分为正常对照组、高糖诱导组(25 mg/mL葡萄糖)、甘露醇对照组(25... |
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| SubjectTerms | E钙黏素 α平滑肌肌动蛋白 丹参 腹膜间皮细胞 过氧化损伤 |
| Title | 丹参注射液对高糖诱导的大鼠腹膜间皮细胞的过氧化损伤及E钙黏素和α平滑肌肌动蛋白表达的影响 |
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