Carboxyl-modified single-walled carbon nanotubes selectively induce human telomeric i-motif formation

As the leading nanodevice candidate, single-walled carbon nanotubes (SWNTs) have potential therapeutic applications in gene therapy and novel drug delivery. We found that SWNTs can inhibit DNA duplex association and selectively induce human telomeric i-motif DNA formation by binding to the 5'-e...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 103; no. 52; pp. 19658 - 19663
Main Authors Li, Xi, Peng, Yinghua, Ren, Jinsong, Qu, Xiaogang
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 26.12.2006
National Acad Sciences
Subjects
Carbon
Chemical bases
Contour lines
Deoxyribonucleic acid
DNA
DNA - chemistry
Fluorescence
Gene therapy
Humans
Hydrogen-Ion Concentration
Melting
Molecular Structure
Molecules
Nanotubes
Nanotubes, Carbon - chemistry
Oligomers
Physical Sciences
Spectrum Analysis
Stellar spectral lines
Studies
Telomere - chemistry
Transition Temperature
Wavelengths
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Summary:As the leading nanodevice candidate, single-walled carbon nanotubes (SWNTs) have potential therapeutic applications in gene therapy and novel drug delivery. We found that SWNTs can inhibit DNA duplex association and selectively induce human telomeric i-motif DNA formation by binding to the 5'-end major groove under physiological conditions or even at pH 8.0. SWNT binding to telomeric DNA was studied by UV melting, NMR, S1 nuclease cleavage, CD, and competitive FRET methods. These results suggest that SWNTs might have the intriguing potential to modulate human telomeric DNA structures in vivo, like biologically relevant B-A and B-Z DNA transitions, which is of great interest for drug design and cancer therapy.
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Edited by Donald M. Crothers, Yale University, New Haven, CT, and approved October 26, 2006
Author contributions: X.L. and Y.P. contributed equally to this work; J.R. and X.Q. designed research; X.L. and Y.P. performed research; X.L. and Y.P. analyzed data; and J.R. and X.Q. wrote the paper.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0607245103