isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast

• Published in: Theoretical and applied genetics Vol. 125; no. 5; pp. 1047 - 1055
• Main Authors: Hua, Lixia, Wu, Jianzhong, Chen, Caixia, Wu, Weihuai, He, Xiuying, Lin, Fei, Wang, Li, Ashikawa, Ikuo, Matsumoto, Takashi, Wang, Ling, Pan, Qinghua
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.09.2012; Springer; Springer Nature B.V
• Subjects: Agriculture; alleles; Biochemistry; Biomedical and Life Sciences; Biotechnology; blast disease; Chromosome Mapping; Chromosomes, Plant - genetics; Development and progression; Diseases and pests; DNA, Plant - genetics; Genes; Genes, Plant - genetics; Genetic aspects; Germplasm; germplasm conservation; Immunity, Innate - genetics; Life Sciences; loci; Magnaporthe - genetics; Magnaporthe - immunology; Magnaporthe - pathogenicity; Magnaporthe oryzae; Original Paper; Oryza - genetics; Oryza - immunology; Oryza - microbiology; Oryza sativa; Pathogens; Physiological aspects; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Plant resistance; Polymerase Chain Reaction; Polymorphism, Single Nucleotide - genetics; Quantitative Trait Loci - genetics; Rice; Rice blast disease; surveys; Japan; China; Rice Blast; Durable Resistance; Race Specificity; Blast Isolate; Blast Resistance
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-012-1894-7

We report the isolation of Pi1, a gene conferring broad-spectrum resistance to rice blast (Magnaporthe oryzae). Using loss- and gain-of-function approaches, we demonstrate that Pi1 is an allele at the Pik locus. Like other alleles at this locus, Pi1 consists of two genes. A functional nucleotide polymorphism (FNP) was identified that allows differentiation of Pi1 from other Pik alleles and other non-Pik genes. A extensive germplasm survey using this FNP reveals that Pi1 is a rare allele in germplasm collections and one that has conferred durable resistance to a broad spectrum of pathogen isolates.