Effects of Oakmoss Components on Extra- and Intracellular Legionella pneumophila and Its Host Acanthamoeba castellanii

• Published in: Biocontrol Science Vol. 27; no. 1; pp. 21 - 29
• Main Authors: NOMURA, HARUE, SUDA, NOBUMASA, KAWANO, YASUYO, ISSHIKI, YASUNORI, SAKUDA, KEISUKE, SAKUMA, KATSUYA, KONDO, SEIICHI
• Format: Journal Article
• Language: English
• Published: Japan The Society for Antibacterial and Antifungal Agents, Japan 01.01.2022; Japan Science and Technology Agency
• Subjects: Acanthamoeba castellanii; Amoeba; Amoebicidal activity; Antibacterial activity; Antibacterial agents; Antibiotics; Antiinfectives and antibacterials; Bacteria; Components; Disinfectants; Humans; Intracellular; Legionella; Legionella pneumophila; Legionnaires' disease bacterium; Oakmoss; Pathogens; Resins, Plant - pharmacology; Terpenes - pharmacology; Antibacterial activity; Legionella pneumophila; Oakmoss; Acanthamoeba castellanii; Amoebicidal activity
• DOI: 10.4265/bio.27.21
• ISSN: 1342-4815

Acanthamoeba castellanii is a ubiquitous organism found in environmental water. The amoeba is pathogenic to toward humans and is also a reservoir of bacteria of the genus Legionella, a causative agent of legionellosis. Oakmoss, a source of natural fragrance ingredients, and its components are antibacterial agents that are specifically active against the genus Legionella. In the present study, oakmoss and its components were investigated for their inhibitory effects on total (extra- and intracellular) Legionella pneumophila within A. castellanii and on L. pneumophila within A. castellanii. Among the oakmoss components, 3-hydroxy-5-methylphenyl 2,4-dihydroxy-6-methylbenzoate (1), 3-methoxy-5-methylphenyl 2,4-dihydroxy-6-methylbenzoate (2), and 8-(2,4-dihydroxy-6-(2-oxoheptyl)phenoxy)-6-hydroxy-3-pentyl-1H-isochromen-1-one (8) reduced the number of total bacteria (extra- and intracellular) in a test culture and also exhibited high amoebicidal activity against L. pneumophila within A. castellanii at concentrations lower than their IC50 values for A. castellanii. In contrast, 6,8-dihydroxy-3-pentyl-1H-isochromen-1-one (5) reduced the total number of L. pneumophila and, also that of total bacteria after 24 h of treatment (P < 0.05), whereas the compound did not exhibit amoebicidal activity against L. pneumophila within A. castellanii at concentrations lower than its IC50 value against A. castellanii. Thus, it is suggested that these oakmoss components could be good candidates for disinfectants to protect from Legionella infection.