The Cellular Retinoic Acid Binding Protein I is Dispensable

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 19; pp. 9032 - 9036
• Main Authors: Gorry, Philippe, Lufkin, Thomas, Dierich, Andree, Rochette-Egly, Cecile, Decimo, Didier, Dolle, Pascal, Mark, Manuel, Durand, Beatrice, Chambon, Pierre
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 13.09.1994; National Acad Sciences; National Academy of Sciences
• Subjects: Alleles; Amino Acid Sequence; Amino acids; Animals; Base Sequence; Biochemistry; Biochemistry, Molecular Biology; Carrier proteins; COS cells; Development Biology; Embryology and Organogenesis; Embryos; Gene Expression; Genes; Genetic mutation; Homozygote; In Situ Hybridization; Life Sciences; Mice; Mice, Knockout - embryology; Molecular Sequence Data; Mutation; Proteins; Receptors, Retinoic Acid - physiology; Retinoids; RNA, Messenger - genetics; Vitamin A
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.91.19.9032

The cellular retinoic acid binding proteins I and II (CRABPI and CRABPII) bind retinoic acid with high affinity, exhibit distinct patterns of expression during embryonic development, and are thought to play important roles in the RA signaling pathway. We have generated a targeted mutation of the CRABPI gene using the "hit-and-run" strategy and shown that it prevents the production of a functional CRABPI protein. Homozygous mutant mice were normal, indicating that CRABPI does not play a crucial role in the RA signaling pathway