Correlation of genetic polymorphism of vascular endothelial growth factor gene with susceptibility to lung cancer

• Published in: Cancer gene therapy Vol. 22; no. 6; pp. 312 - 316
• Main Authors: Liu, C, Zhou, X, Gao, F, Qi, Z, Zhang, Z, Guo, Y
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2015; Nature Publishing Group
• Subjects: 45/23; 692/699; Adult; Aged; Alleles; Asian Continental Ancestry Group; Biomedical and Life Sciences; Biomedicine; Case-Control Studies; Development and progression; Disease susceptibility; Female; Gene Expression; Gene polymorphism; Gene Therapy; Genetic aspects; Genetic polymorphisms; Genetic Predisposition to Disease; Haplotypes; Health aspects; Humans; Leukocytes; Lung cancer; Lung Neoplasms - genetics; Male; Middle Aged; original-article; Polymerase chain reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Restriction fragment length polymorphism; Single-nucleotide polymorphism; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - genetics
• ISSN: 0929-1903; 1476-5500; 1476-5500
• DOI: 10.1038/cgt.2015.24

The aim of the study is to study the correlation of genetic polymorphism of vascular endothelial growth factor (VEGF) gene with susceptibility to primary lung cancer. A total of 414 patients with primary lung cancer and 338 healthy volunteers were enrolled in this case–control study from September 2008 to October 2011. Gene identification with PCR-RFLP (polymerase chain reaction-based restriction fragment length polymorphism) was used to detect in white blood cells from the subjects the single-nucleotide polymorphisms (SNP) of VEGF gene, including +405G/C, −460 T/C, −1154G/A, −2578C/A sites. Association of genotypes or haplotypes with susceptibility of lung cancer was analyzed with unconditional logistic regression adjusted by gender and age. Smoking was significantly associated with increased risk of lung cancer. Gene phenotypic analysis demonstrated that C allele of +405G/C in VEGF gene was significantly associated increased risk of lung cancer in males ( P =0.0094, odds ratio=1.634.3), as that with carrying GCTC haplotype (odds ratio=1.349), whereas carrying GACG had decreased risk for lung cancer (odds ratio=0.044). No relationship existed between 460 T/C, −1154G/A, −2578C/A alleles of VEGF gene and risk of lung cancer. VEGF gene polymorphism may have a role in the development of lung cancer.