Neural stem cells over-expressing brain-derived neurotrophic factor promote neuronal survival and cytoskeletal protein expression in traumatic brain injury sites

• Published in: Neural regeneration research Vol. 12; no. 3; pp. 433 - 439
• Main Authors: Chen, Tao, Yu, Yan, Tang, Liu-jiu, Kong, Li, Zhang, Cheng-hong, Chu, Hai-ying, Yin, Liang-wei, Ma, Hai-ying
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.03.2017; Medknow Publications & Media Pvt. Ltd; Department of Neurosurgery,the First Affiliated Hospital of Dalian Medical University,Dalian,Liaoning Province,China%Department of Histology and Embryology,Dalian Medical University,Dalian,Liaoning Province,China 3 Department of Oncology,Dalian Central Hospital,Dalian,Liaoning Province,China%Department of Oncology,Dalian Central Hospital,Dalian,Liaoning Province,China; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Brain injuries; Brain-derived neurotrophic factor; Care and treatment; Cytoskeletal proteins; Growth factors; Health aspects; Laboratories; nerve regeneration; brain-derived neurotrophic factor; neural stem cells; transfect; differentiation; traumatic brain injury; cytoskeleton; neurofilament; microtubule-associated proteins; calmodulin; Wnt/β-catenin; neural regeneration; Nervous system; Neurons; Physiological aspects; Protein expression; Proteins; Signal transduction; Stem cell transplantation; Stem cells; Transplants & implants; Traumatic brain injury; 存活; 损伤部位; 神经元; 神经干细胞; 细胞骨架蛋白; 脑源性神经营养因子; 蛋白表达; 过度表达; China; United States > US; differentiation; neural regeneration; cytoskeleton; neural stem cells; neurofilament; transfect; traumatic brain injury; calmodulin; Wnt/β-catenin; nerve regeneration; brain-derived neurotrophic factor; microtubule-associated proteins; Wnt/β-catenin; calmodulin
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.202947

Cytoskeletal proteins are involved in neuronal survival.Brain-derived neurotrophic factor can increase expression of cytoskeletal proteins during regeneration after axonal injury.However,the effect of neural stem cells genetically modified by brain-derived neurotrophic factor transplantation on neuronal survival in the injury site still remains unclear.To examine this,we established a rat model of traumatic brain injury by controlled cortical impact.At 72 hours after injury,2 × 10~7 cells/m L neural stem cells overexpressing brain-derived neurotrophic factor or naive neural stem cells（3 m L） were injected into the injured cortex.At 1–3 weeks after transplantation,expression of neurofilament 200,microtubule-associated protein 2,actin,calmodulin,and beta-catenin were remarkably increased in the injury sites.These findings confirm that brain-derived neurotrophic factor-transfected neural stem cells contribute to neuronal survival,growth,and differentiation in the injury sites.The underlying mechanisms may be associated with increased expression of cytoskeletal proteins and the Wnt/β-catenin signaling pathway.