Bovine respiratory syncytial virus enhances the attachment of Trueperella pyogenes to cells

• Published in: Journal of Veterinary Medical Science Vol. 86; no. 10; pp. 1068 - 1075
• Main Authors: YAMAMOTO, Satomi, OKUMURA, Shiori, KOBAYASHI, Risa, MAEDA, Yosuke, TAKAHASHI, Fumiaki, TANABE, Taishi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2024; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: bacterial adhesion; Bacterial infections; bovine respiratory disease complex; bovine respiratory syncytial virus; Cattle; co-infection; Gentamicin; Immunofluorescence; Infections; Multiplicity of infection; Proteins; Respiratory diseases; Respiratory syncytial virus; Trueperella pyogenes; Viral infections; Virology; co-infection; bovine respiratory disease complex; Trueperella pyogenes; bacterial adhesion; bovine respiratory syncytial virus
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.24-0068

In cattle, bovine respiratory syncytial virus (BRSV) is associated with secondary bacterial infections; however, the mechanisms of the interaction between BRSV and bacteria are unclear. Trueperella pyogenes (T. pyogenes) causes pneumonia in cattle and is involved in secondary infections following viral infections. In this study, we evaluated the effect of BRSV infection on the adhesion of T. pyogenes to BRSV-infected cells. BRSV infection significantly enhanced the adhesion of T. pyogenes to cells in a multiplicity of infection- and time-dependent manner. The BRSV-mediated change in the adhesion of T. pyogenes was widely observed in various cell types and bacterial strains. The results from the gentamicin protection assay showed that BRSV infection did not affect the intracellular invasion ability of T. pyogenes. Furthermore, adhesion assays conducted using BRSV G protein-expressing cells and anti-BRSV G antibodies revealed that the increased adhesion of T. pyogenes to cells was mediated by the G protein of BRSV. In addition, immunofluorescence assay revealed the colocalization of BRSV G protein and T. pyogenes. Thus, BRSV infection can potentially lead to bovine respiratory disease complex by promoting the adhesion of T. pyogenes to the infected cells.