Radiosynthesis of 6'-Deoxy-6'[18F]Fluorosucrose via Automated Synthesis and Its Utility to Study In Vivo Sucrose Transport in Maize (Zea mays) Leaves

• Published in: PloS one Vol. 10; no. 5; p. e0128989
• Main Authors: Rotsch, David, Brossard, Tom, Bihmidine, Saadia, Ying, Weijiang, Gaddam, Vikram, Harmata, Michael, Robertson, J David, Swyers, Michael, Jurisson, Silvia S, Braun, David M
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.05.2015; Public Library of Science (PLoS)
• Subjects: Automation; Biological Transport, Active - physiology; Carbohydrates; Chemical synthesis; Corn; ENVIRONMENTAL SCIENCES; Flowers; Fluorine; Fluorine Radioisotopes - pharmacokinetics; Fluorine Radioisotopes - pharmacology; Half-life; high performance liquid chromatography; Hydrocarbons, Fluorinated - chemical synthesis; Hydrocarbons, Fluorinated - chemistry; Hydrocarbons, Fluorinated - pharmacokinetics; Hydrocarbons, Fluorinated - pharmacology; In vivo methods and tests; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Interdisciplinary aspects; Isotope Labeling; leaf veins; Leaves; maize; Monosaccharide Transport Proteins - metabolism; Organs; Phloem; Photosynthesis; Physiology; Plant biochemistry; Plant Leaves - metabolism; Plant Proteins - metabolism; Process controls; Proteins; Radioactive half-life; Radioactive tracers; Radioisotopes; Science; Seeds; solute transport; Studies; Sucrose; Sucrose - analogs & derivatives; Sucrose - chemical synthesis; Sucrose - chemistry; Sucrose - pharmacokinetics; Sucrose - pharmacology; Sugar; SUT1 gene; Tissues; Tracers (Chemistry); Transport; Uranium; Veins (plant anatomy); Zea mays; Zea mays - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0128989

Sugars produced from photosynthesis in leaves are transported through the phloem tissues within veins and delivered to non-photosynthetic organs, such as roots, stems, flowers, and seeds, to support their growth and/or storage of carbohydrates. However, because the phloem is located internally within the veins, it is difficult to access and to study the dynamics of sugar transport. Radioactive tracers have been extensively used to study vascular transport in plants and have provided great insights into transport dynamics. To better study sucrose partitioning in vivo, a novel radioactive analog of sucrose was synthesized through a completely chemical synthesis route by substituting fluorine-18 (half-life 110 min) at the 6' position to generate 6'-deoxy-6'[(18)F]fluorosucrose ((18)FS). This radiotracer was then used to compare sucrose transport between wild-type maize plants and mutant plants lacking the Sucrose transporter1 (Sut1) gene, which has been shown to function in sucrose phloem loading. Our results demonstrate that (18)FS is transported in vivo, with the wild-type plants showing a greater rate of transport down the leaf blade than the sut1 mutant plants. A similar transport pattern was also observed for universally labeled [U-(14)C]sucrose ([U-(14)C]suc). Our findings support the proposed sucrose phloem loading function of the Sut1 gene in maize, and additionally demonstrate that the (18)FS analog is a valuable, new tool that offers imaging advantages over [U-(14)C]suc for studying phloem transport in plants.