Neuroprotective effects of ganoderma lucidum polysaccharides against oxidative stress-induced neuronal apoptosis

• Published in: Neural regeneration research Vol. 12; no. 6; pp. 953 - 958
• Main Authors: Sun, Xin-zhi, Liao, Ying, Li, Wei, Guo, Li-mei
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.06.2017; Medknow Publications & Media Pvt. Ltd; Department of Pathology, Peking University Health Science Center, Beijing, China%Department of Public Security Technology, Railway Police College, Zhengzhou, Henan Province, China%Department of Pathology, Peking University Health Science Center, Beijing, China; Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China%Department of Public Security Technology, Railway Police College, Zhengzhou, Henan Province, China; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Alzheimer's disease; Apoptosis; caspase-3; Cytochrome; Experiments; Health aspects; Immunoglobulins; Kinases; Laboratory animals; nerve regeneration; brain injury; H2O2; cerebellar granule cells; Bim; Bax; Bcl-2; cytochrome C; caspase-3; neural regeneration; Neurons; Oxidative stress; Physiological aspects; Polysaccharides; Properties; Proteins; Reishi; Signal transduction; Spinal cord injuries; 凋亡; 小脑颗粒细胞; 氧化应激; 灵芝多糖; 神经保护作用; 神经元; 诱导; United States > US; China; Bcl-2; brain injury; neural regeneration; Bim; Bax; cerebellar granule cells; nerve regeneration; H2O2; cytochrome C; caspase-3
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.208590

Ganoderma lucidum polysaccharides have protective effects against apoptosis in neurons exposed to ischemia/reperfusion injury, but the mechanisms are unclear. The goal of this study was to investigate the underlying mechanisms of the effects of ganoderma lucidum polysaccharides against oxidative stress-induced neuronal apoptosis. Hydrogen peroxide（H_2O_2） was used to induce apoptosis in cultured cerebellar granule cells. In these cells, ganoderma lucidum polysaccharides remarkably suppressed H_2O_2-induced apoptosis, decreased expression of caspase-3, Bax and Bim and increased that of Bcl-2. These findings suggested that ganoderma lucidum polysaccharides regulate expression of apoptosis-associated proteins, inhibit oxidative stress-induced neuronal apoptosis and, therefore, have significant neuroprotective effects.