Characterization of a Corrinoid Compound from Pacific Bluefin Tuna (Thunnus orientalis) Liver
A substantial amount of vitamin B12 (357 ± 42.7 μg/100 g) was detected in pacific bluefin tuna (Thunnus orientalis) liver using a microbiological assay method. To evaluate whether tuna liver contains true vitamin B12 or an inactive corrinoid compound, a corrinoid compound was purified and characteri...
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Published in | FOOD SCIENCE AND TECHNOLOGY RESEARCH Vol. 17; no. 6; pp. 589 - 594 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English Japanese |
Published |
Tsukuba
Japanese Society for Food Science and Technology
2011
The Japanese Society for Food Science and Technology Japan Science and Technology Agency |
Subjects | |
Online Access | Get full text |
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Summary: | A substantial amount of vitamin B12 (357 ± 42.7 μg/100 g) was detected in pacific bluefin tuna (Thunnus orientalis) liver using a microbiological assay method. To evaluate whether tuna liver contains true vitamin B12 or an inactive corrinoid compound, a corrinoid compound was purified and characterized. The purified compound was identical to that of authentic vitamin B12, judging by TLC and LC/ESI-MS/MS chromatograms, and 1H NMR spectral data. These results indicate that pacific bluefin tuna liver contains substantial amounts of true vitamin B12 and would be an excellent source of the vitamin. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1344-6606 1881-3984 |
DOI: | 10.3136/fstr.17.589 |