Disruption of ptsG gene and manXYZ operon of ethanol-producing Escherichia coli KO11: Effects on glucose and xylose utilization and ethanol production

• Published in: Journal of bioscience and bioengineering Vol. 113; no. 5; pp. 608 - 610
• Main Authors: Ohta, Kazuyoshi, Hamasuna, Hironori, Tsukamoto, Junji, Wakiyama, Motoki, Izumi, Yoshiya, Harada, Keiko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2012; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; Deletion mutant; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli KO11; Ethanol - metabolism; Ethanol fermentation; ethanol production; Fermentation; Fundamental and applied biological sciences. Psychology; Genes, Bacterial - genetics; glucose; Glucose - metabolism; manXYZ; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; mutants; operon; Operon - genetics; Phosphoenolpyruvate Sugar Phosphotransferase System - genetics; ptsG; Sequence Deletion; Time Factors; Xylose; Xylose - metabolism; Xylose; Ethanol fermentation; ptsG; manXYZ; Escherichia coli KO11; Ethanol; Operon; Escherichia coli; Glucose; Fermentation; Gene; Production; Bacteria; Enterobacteriaceae
• ISSN: 1389-1723; 1347-4421; 1347-4421
• DOI: 10.1016/j.jbiosc.2011.12.020

Ethanol-producing Escherichia coli strain KO11 consumed 99% of the glucose and only 13% of the xylose in a mixture of glucose (60g/L) and xylose (40g/L) during the 72-h fermentation at 30°C. The deletion mutants ΔptsG, ΔmanXYZ, and ΔptsG/manXYZ utilized 42%, 78%, and 35% of the glucose and 50%, 32%, and 32% of the xylose, respectively.