Low-molecular-weight fucoidan and high-stability fucoxanthin from brown seaweed exert prebiotics and anti-inflammatory activities in Caco-2 cells

• Published in: Food & nutrition research Vol. 60; no. 1; pp. 32033 - 9
• Main Authors: Hwang, Pai-An, Phan, Nam Nhut, Lu, Wen-Jung, Hieu, Bui Thi Ngoc, Lin, Yen-Chang
• Format: Journal Article
• Language: English
• Published: Sweden Taylor & Francis 01.01.2016; Swedish Nutrition Foundation, SNF; Co-Action Publishing; Swedish Nutrition Foundation
• Subjects: Algae; anti-inflammatory; Anti-inflammatory agents; antiinflammatoriske midler; Assaying; Bifidobacterium lactis; Carotenoids; Cell culture; Cytokines; Epithelial cells; Food; Fucoidan; Fucoxanthin; Gene expression; IL-1β; Immune response; Immune system; Inflammation; Interleukin 10; Intestine; Lipopolysaccharides; Low molecular weights; low-molecular-weight fucoidan; Lymphocytes B; Morinda citrifolia; Original; Prebiotics; prebiotika; Probiotics; Proteins; Rodents; Sargassum; Seaweeds; Small intestine; Stability; tang; Tumor necrosis factor-α; γ-Interferon; Taiwan; anti-inflammatory; prebiotics; low-molecular-weight fucoidan; fucoxanthin
• ISSN: 1654-6628; 1654-661X; 1654-661X
• DOI: 10.3402/fnr.v60.32033

The aim of this study is to investigate the anti-inflammatory effects of low-molecular-weight fucoidan (LMF) and high-stability fucoxanthin (HS-Fucox) in a lipopolysaccharide-induced inflammatory Caco-2 cell line co-culture with B. lactis. We used various methods such as transepithelial resistance (TER) assay, cytokine secretion assay, and tight junction protein mRNA expression assay to examine LMF and HS-Fucox anti-inflammatory properties. LMF and HS-Fucox activated probiotic growth and reduced the inflammation of the intestinal epithelial cells. Moreover, the combination of LMFHS-Fucox dramatically enhanced the intestinal epithelial barrier and immune function against the lipopolysaccharide effect by inhibiting IL-1β and TNF-α and promoting IL-10 and IFN-γ. These findings suggested that LMF and HS-Fucox, alone or in combination, could be the potential natural compounds to enhance the immune system and have an anti-inflammatory effect on the intestinal cells.