Screening and incorporation of rust resistance from Allium cepa into bunching onion (Allium fistulosum) via alien chromosome addition

• Published in: Genome Vol. 58; no. 4; pp. 135 - 142
• Main Authors: Wako, Tadayuki, Yamashita, Ken-ichiro, Tsukazaki, Hikaru, Ohara, Takayoshi, Kojima, Akio, Yaguchi, Shigenori, Shimazaki, Satoshi, Midorikawa, Naoko, Sakai, Takako, Yamauchi, Naoki, Shigyo, Masayoshi
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.04.2015; Canadian Science Publishing NRC Research Press
• Subjects: Allium cepa; Allium fistulosum; Basidiomycota - immunology; Basidiomycota - physiology; Breeding; bunching onion; Chromosomes; Chromosomes, Plant - genetics; ciboule; Control; Cultivars; Disease Resistance - genetics; Fungi; Genes; Genetic aspects; Genetic screening; Health aspects; lignées d’addition monosomiques; Methods; monosomic addition lines; Onions; Onions - genetics; Onions - immunology; Onions - microbiology; Plant Diseases - immunology; Plant Diseases - microbiology; Plant immunology; Plant Leaves - genetics; Plant Leaves - immunology; Plant Leaves - microbiology; Plant-pathogen relationships; Puccinia allii; Rust fungi; rust resistance; Seedlings; Seedlings - genetics; Seedlings - immunology; Seedlings - microbiology; shallot; Shallots - genetics; Shallots - immunology; Shallots - microbiology; Vegetables; échalote; ésistance à la rouille; Allium cepa; monosomic addition lines; ciboule; lignées d’addition monosomiques; échalote; ésistance à la rouille; Allium fistulosum; rust resistance; bunching onion; shallot
• ISSN: 1480-3321; 0831-2796; 1480-3321
• DOI: 10.1139/gen-2015-0026

Bunching onion (Allium fistulosum L.; 2n = 16), bulb onion (Allium cepa L. Common onion group), and shallot (Allium cepa L. Aggregatum group) cultivars were inoculated with rust fungus, Puccinia allii, isolated from bunching onion. Bulb onions and shallots are highly resistant to rust, suggesting they would serve as useful resources for breeding rust resistant bunching onions. To identify the A. cepa chromosome(s) related to rust resistance, a complete set of eight A. fistulosum – shallot monosomic alien addition lines (MAALs) were inoculated with P. allii. At the seedling stage, FF+1A showed a high level of resistance in controlled-environment experiments, suggesting that the genes related to rust resistance could be located on shallot chromosome 1A. While MAAL, multi-chromosome addition line, and hypoallotriploid adult plants did not exhibit strong resistance to rust. In contrast to the high resistance of shallot, the addition line FF+1A+5A showed reproducibly high levels of rust resistance.