Antibacterial efficacy, corrosion resistance, and cytotoxicity studies of copper-substituted carbonated hydroxyapatite coating on titanium substrate

• Published in: Journal of materials science Vol. 50; no. 4; pp. 1688 - 1700
• Main Authors: Huang, Yong, Zhang, Xuejiao, Zhao, Ranlin, Mao, Huanhuan, Yan, Yajing, Pang, Xiaofeng
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.02.2015; Springer; Springer Nature B.V
• Subjects: Analysis; Antibacterial agents; Apatite; Bacteria; Biocompatibility; Biomedical materials; Body fluids; Carbonates; Carbonation; Characterization and Evaluation of Materials; Chemistry and Materials Science; Classical Mechanics; Coating; COATINGS; Coatings industry; Copper; CORROSION; Corrosion and anti-corrosives; Corrosion prevention; CORROSION PROTECTION; Corrosion resistance; Crystallography and Scattering Methods; Crystals; Cytotoxicity; Diffraction; E coli; ELECTROPLATING; Energy dispersive X ray spectroscopy; Escherichia coli; Fourier transforms; Hydroxyapatite; In vitro methods and tests; Infrared analysis; Infrared spectroscopy; Materials Science; MICRO ORGANISMS; Nucleation; Original Paper; Phosphate minerals; Phosphate rock; Polymer Sciences; Porosity; Protective coatings; Scanning electron microscopy; Solid Mechanics; Spectrum analysis; Substitutes; Substrates; Surgical implants; Titanium; TOXICITY; X ray analysis; X-rays; Simulated Body Fluid; Cupric Nitrate; Antibacterial Efficacy; Simulated Body Fluid Immersion; Electrolytic Deposition
• ISSN: 0022-2461; 1573-4803
• DOI: 10.1007/s10853-014-8730-1

This work elucidated the antibacterial efficacy, corrosion resistance, and cytotoxicity of electroplated copper-substituted hydroxyapatite (CuHAP) coating on titanium (Ti). The fabricated CuHAP coatings were characterized by scanning electron microscopy, energy-dispersive X-ray analysis spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction studies. The CuHAP coating had needle-like apatite crystals, the doping of Cu 2+ into HAP reduced porosity, and the coating became denser. The CuHAP crystals were carbonated with a few of Cu 2+ incorporation (about 0.80 wt%). The Cu 2+ ions were homogenously deposited into HAP films. Potentiodynamic polarisation test revealed that the CuHAP coating provided good barrier characteristics and achieved superior corrosion protection for Ti substrates. The in vitro antibacterial activity of as-prepared CuHAP coating was evaluated against Escherichia coli and was found to be effectively high against bacterial colonization. Bioactivity test conducted by soaking the coatings in simulated body fluid demonstrated that CuHAP coating can quickly induce bone-like apatite nucleation and growth. In vitro biocompatibility tests, MTT, were employed to assess the cytotoxicity of CuHAP coating with osteoblast-like MC3T3-E1 cells. The obtained HAP coating doped with a low content of Cu 2+ exhibited good cytocompatibility and had no toxicity toward MC3T3-E1.