Characterization of an Arabidopsis thaliana receptor-like protein kinase gene activated by oxidative stress and pathogen attack

• Published in: The Plant journal : for cell and molecular biology Vol. 18; no. 3; pp. 321 - 327
• Main Authors: Czernic, P, Visser, B, Sun, W, Savoure, A, Deslandes, L, Marco, Y, Van Montagu, M, Verbruggen, N
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.05.1999; Blackwell Science
• Subjects: Amino Acid Sequence; amino acid sequences; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - microbiology; Arabidopsis thaliana; Bacterial plant pathogens; Base Sequence; Biological and medical sciences; compatibility; complementary DNA; cultured cells; DNA Primers - genetics; DNA, Complementary - genetics; DNA, Plant - genetics; experimental infections; flowers; Fundamental and applied biological sciences. Psychology; genbank/100670; genbank/122302; genbank/913140 [sic]; genbank/aj011674; gene expression; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Plant - drug effects; genes; Genes, Plant; genetic techniques and protocols; leaves; messenger RNA; Molecular Sequence Data; nucleotide sequences; oxidation; Oxidative Stress; Pathology. Damages, economic importance; Phytopathology. Animal pests. Plant and forest protection; Protein Kinases - genetics; Pseudomonas - pathogenicity; Pseudomonas syringae pv. tomato; Ralstonia solanacearum; receptors; roots; salicylic acid; Salicylic Acid - pharmacology; Sequence Homology, Amino Acid; stems; stress; Tissue Distribution; Cell culture; Salicylic acid; Oxidative stress; Enzyme; Transferases; Tissue specificity; Host agent relation; Activation; Gene expression; Arabidopsis thaliana; Signal transduction; Complementary DNA; Gene; Cruciferae; Protein kinase; Dicotyledones; Angiospermae; Spermatophyta; Experimental plant; Sequencing
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.1999.00447.x

An Arabidopsis thaliana cDNA clone that encodes a putative receptor-like protein kinase gene (At-RLK3) was characterized. The deduced 667-amino acid protein consists of an amino-terminal signal sequence, an extracellular domain, a single transmembrane domain, and a cytoplasmic domain with characteristics of serine/threonine protein kinase. Because of the original features of its extracellular domain, the At-RLK3 protein is a member of a new class of receptor-like protein kinases. The At-RLK3 gene is present as a single copy within the Arabidopsis genome and its transcripts are detected in root, stem, leaf and flower. In cultured cells, the At-RLK3 gene is activated upon oxidative stress and salicylic acid treatment. ln plants, the gene appears to be differentially regulated during various plant-pathogen interactions: upon inoculation with strains of Pseudomonas syringae pv. tomato harboring or not, different avr genes, At-RLK3 transcripts accumulate transiently at similar levels during both compatible and incompatible interactions. This gene is, however, preferentially expressed during the incompatible interaction induced by the soil-borne vascular bacteria, Ralstonia solanacearum. The involvement of At-RLK3 in signal transduction pathways during pathogen attack is discussed.