Broad specificity of monoclonal IgA (TEPC15-IgA) for enteric bacteria via phosphorylcholine-mediated interaction

Immunoglobulin A (IgA) is notable for its broad specificity toward multiple bacteria. Phosphorylcholine (PC) plays a role in the infection of pathogenic bacteria carrying PC and in the induction of IgA responses in the host immune system. The commercially available mouse monoclonal IgA, TEPC15-IgA,...

Full description

Saved in:
Bibliographic Details
Published inJournal of Veterinary Medical Science Vol. 86; no. 7; pp. 801 - 808
Main Authors KOYAMA, Saeka, ITO, Kaori, USAMI, Katsuki, WADA, Shino, YAMASHITA, Tsukasa, IKEDA-OHTSUBO, Wakako, KITAZAWA, Haruki, HIRAKAWA, Ryota, ISLAM, Jahidul, FURUKAWA, Mutsumi, NOCHI, Tomonori
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2024
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Bacteria
enteric bacteria
Enterobacteriaceae - immunology
Gastrointestinal tract
Immune system
Immunoglobulin A
Immunoglobulin A - immunology
Immunology
Lactobacillus
Mice
Mice, Inbred BALB C
Microflora
Molecular weight
Phosphorylcholine
Phosphorylcholine - immunology
immune system
immunoglobulin A
enteric bacteria
phosphorylcholine
Online AccessGet full text

Cover

More Information
Summary:Immunoglobulin A (IgA) is notable for its broad specificity toward multiple bacteria. Phosphorylcholine (PC) plays a role in the infection of pathogenic bacteria carrying PC and in the induction of IgA responses in the host immune system. The commercially available mouse monoclonal IgA, TEPC15-IgA, is a distinctive antibody with specificity for PC, warranting further exploration of its response to PC-bearing enteric bacteria. In this study, using 17 different enteric bacteria, including 3 aerobic and 14 anerobic bacteria that could be cultured in vitro, we confirmed that TEPC15-IgA recognizes 4 bacterial species: Lactobacillus taiwanensis, Limosilactobacillus frumenti, Streptococcus infantis, and Escherichia coli, although reactivity varied. Interestingly, TEPC15-IgA did not react with four of six Lactobacillus species used. Moreover, distinct target molecules associated with PC in L. taiwanensis and L. frumenti were evident, differing in molecular weight. These findings suggest that the natural generation of PC-specific IgA could prevent PC-mediated infections and potentially facilitate the formation of a microflora rich in indigenous bacteria with PC, particularly in the gastrointestinal tract.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:0916-7250
1347-7439
1347-7439
DOI:10.1292/jvms.23-0441