Impaired Thiol-Disulfide Balance in Acute Brucellosis

• Published in: Japanese Journal of Infectious Diseases Vol. 70; no. 3; pp. 258 - 262
• Main Authors: Kolgelier, Servet, Ergin, Merve, Demir, Lutfi Saltuk, Inkaya, Ahmet Cagkan, Demir, Nazlim Aktug, Alisik, Murat, Erel, Ozcan
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 2017; Japan Science and Technology Agency
• Subjects: acute brucellosis; Adult; Antioxidants; Blood Chemical Analysis - methods; Brucellosis; Brucellosis - pathology; Ceruloplasmin; disulfide/total thiol ratio; Disulfides - blood; Female; Homeostasis; Humans; Inflammation; Male; Middle Aged; oxidative stress; Parameters; Patients; Sulfhydryl Compounds - blood; thiol-disulfide homeostasis; thiol-disulfide homeostasis; acute brucellosis; inflammation; disulfide/total thiol ratio; oxidative stress
• ISSN: 1344-6304; 1884-2836
• DOI: 10.7883/yoken.JJID.2016.196

The objective of this study was to examine a novel profile: thiol-disulfide homeostasis in acute brucellosis. The study included 90 patients with acute brucellosis, and 27 healthy controls. Thiol-disulfide profile tests were analyzed by a recently developed method, and ceruloplasmin levels were determined. Native thiol levels were 256.72 ± 48.20 μmol/L in the acute brucellosis group and 461.13 ± 45.37 μmol/L in the healthy group, and total thiol levels were 298.58 ± 51.78 μmol/L in the acute brucellosis group and 504.83 ± 51.05 μmol/L in the healthy group (p < 0.001, for both). The disulfide/native thiol ratios and disulfide/total thiol ratios were significantly higher, and native thiol/total thiol ratios were significantly lower in patients with acute brucellosis than in the healthy controls (p < 0.001, for all ratios). There were either positive or negative relationships between ceruloplasmin levels and thiol-disulfide parameters. The thiol-disulfide homeostasis was impaired in acute brucellosis. The strong associations between thiol-disulfide parameters and a positive acute-phase reactant reflected the disruption of the balance between the antioxidant and oxidant systems. Since thiol groups act as anti-inflammatory mediators, the alteration in the thiol-disulfide homeostasis may be involved in brucellosis.