CD133 (Prominin) negative human neural stem cells are clonogenic and tripotent

• Published in: PloS one Vol. 4; no. 5; p. e5498
• Main Authors: Sun, Yirui, Kong, Weiqing, Falk, Anna, Hu, Jin, Zhou, Liangfu, Pollard, Steve, Smith, Austin
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11.05.2009; Public Library of Science (PLoS)
• Subjects: AC133 Antigen; Animals; Antigens, CD - genetics; Antigens, CD - metabolism; B cells; Biochemistry; Biotechnology; Brain; Brain cancer; Brain research; Brain stem; Brain tumors; Cancer; Cell Biology/Cell Growth and Division; Cell Biology/Neuronal and Glial Cell Biology; Cell cycle; Cell growth; Cell Line; Cell Proliferation; Clone Cells - cytology; Clone Cells - metabolism; Colorectal cancer; Cycles; Cytometry; Developmental Biology/Stem Cells; Epidermal growth factor; Flow Cytometry; Fractionation; G1 Phase; Gene Expression; Glycoproteins - genetics; Glycoproteins - metabolism; Humans; Immunohistochemistry; Immunoreactivity; Ki-67 Antigen - analysis; Labeling; Labelling; Lewis X Antigen - analysis; Mammals; Medical research; Melanoma; mRNA; Nervous system; Neural stem cells; Neurons - cytology; Neurons - metabolism; Neurophysiology; Neurosciences; Peptides - genetics; Peptides - metabolism; Rats; Resting Phase, Cell Cycle; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stem cell transplantation; Stem cells; Stem Cells - cytology; Stem Cells - metabolism; Telomerase; Totipotent Stem Cells - cytology; Totipotent Stem Cells - metabolism; Tumors

CD133 (Prominin) is widely used as a marker for the identification and isolation of neural precursor cells from normal brain or tumor tissue. However, the assumption that CD133 is expressed constitutively in neural precursor cells has not been examined. In this study, we demonstrate that CD133 and a...