Observation of dissociative quasi-free electron attachment to nucleoside via excited anion radical in solution

• Published in: Nature communications Vol. 10; no. 1; p. 102
• Main Authors: Ma, Jun, Kumar, Anil, Muroya, Yusa, Yamashita, Shinichi, Sakurai, Tsuneaki, Denisov, Sergey A., Sevilla, Michael D., Adhikary, Amitava, Seki, Shu, Mostafavi, Mehran
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.01.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 101/58; 140/125; 639/638/440/56; 639/638/440/947; Anions; Chemical Sciences; Conduction; Conduction bands; Data analysis; Data processing; Deoxyribonucleic acid; Diethylene glycol; DNA; DNA - chemistry; DNA Damage; Electron attachment; Electron beams; Electrons; Energy; Free Radicals - chemistry; Humanities and Social Sciences; Lasers; multidisciplinary; Nucleosides - chemistry; Radiation; Science; Science (multidisciplinary); Solvents; Time constant
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-08005-z

Damage to DNA via dissociative electron attachment has been well-studied in both the gas and condensed phases; however, understanding this process in bulk solution at a fundamental level is still a challenge. Here, we use a picosecond pulse of a high energy electron beam to generate electrons in liquid diethylene glycol and observe the electron attachment dynamics to ribothymidine at different stages of electron relaxation. Our transient spectroscopic results reveal that the quasi-free electron with energy near the conduction band effectively attaches to ribothymidine leading to a new absorbing species that is characterized in the UV-visible region. This species exhibits a nearly concentration-independent decay with a time constant of ~350 ps. From time-resolved studies under different conditions, combined with data analysis and theoretical calculations, we assign this intermediate to an excited anion radical that undergoes N1-C1′ glycosidic bond dissociation rather than relaxation to its ground state. Radiation-induced low-energy electrons in solution are implicated in DNA damage, but their relaxation dynamics are not well understood. Here the authors observe how quasi-free electrons dissociate glycosidic bonds via an excited nucleoside anion radical, whereas solvated electrons reside on the nucleoside as a relatively stable anion radical.