Fungal burden, dimorphic transition and candidalysin: Role in Candida albicans-induced vaginal cell damage and mitochondrial activation in vitro

• Published in: PloS one Vol. 19; no. 5; p. e0303449
• Main Authors: Spaggiari, Luca, Ardizzoni, Andrea, Ricchi, Francesco, Pedretti, Natalia, Squartini Ramos, Caterina Alejandra, Squartini Ramos, Gianfranco Bruno, Kenno, Samyr, De Seta, Francesco, Pericolini, Eva
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.05.2024; Public Library of Science (PLoS)
• Subjects: Antifungal agents; Biology and Life Sciences; Candida albicans; Candida albicans - metabolism; Candida albicans - physiology; Candidiasis; Candidiasis, Vulvovaginal - microbiology; Cell activation; Cell death; Cell Line; Cells; Chemical properties; Damage; Dimorphism (Biology); Epithelial cells; Epithelial Cells - metabolism; Epithelial Cells - microbiology; Epithelium; Female; Fungal Proteins - metabolism; Fungi; Health aspects; Humans; Hyphae; Hyphae - growth & development; Hyphae - metabolism; Infection; Inflammation; Inflammatory response; Medicine and Health Sciences; Methicillin; Microbiological research; Microbiota; Mitochondria; Mitochondria - metabolism; Morphogenesis; Pathogens; Physical sciences; Physiological aspects; Reactive oxygen species; Reactive Oxygen Species - metabolism; Reading; Research and Analysis Methods; Staphylococcus infections; Vagina; Vagina - microbiology; Virulence; Yeast; Yeasts; California; Italy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0303449

Candida albicans (C. albicans) can behave as a commensal yeast colonizing the vaginal mucosa, and in this condition is tolerated by the epithelium. When the epithelial tolerance breaks down, due to C. albicans overgrowth and hyphae formation, the generated inflammatory response and cell damage lead to vulvovaginal candidiasis (VVC) symptoms. Here, we focused on the induction of mitochondrial reactive oxygen species (mtROS) in vaginal epithelial cells after C. albicans infection and the involvement of fungal burden, morphogenesis and candidalysin (CL) production in such induction. Bioluminescent (BLI) C. albicans, C. albicans PCA-2 and C. albicans 529L strains were employed in an in vitro infection model including reconstituted vaginal epithelium cells (RVE), produced starting from A-431 cell line. The production of mtROS was kinetically measured by using MitoSOX™ Red probe. The potency of C. albicans to induced cell damage to RVE and C. albicans proliferation have also been evaluated. C. albicans induces a rapid mtROS release from vaginal epithelial cells, in parallel with an increase of the fungal load and hyphal formation. Under the same experimental conditions, the 529L C. albicans strain, known to be defective in CL production, induced a minor mtROS release showing the key role of CL in causing epithelial mithocondrial activation. C. albicans PCA-2, unable to form hyphae, induced comparable but slower mtROS production as compared to BLI C. albicans yeasts. By reducing mtROS through a ROS scavenger, an increased fungal burden was observed during RVE infection but not in fungal cultures grown on abiotic surface. Collectively, we conclude that CL, more than fungal load and hyphae formation, seems to play a key role in the rapid activation of mtROS by epithelial cells and in the induction of cell-damage and that mtROS are key elements in the vaginal epithelial cells response to C. albicans.