Humoral response to the central unglycosylated region of the respiratory syncytial virus attachment protein

• Published in: Vaccine Vol. 28; no. 38; pp. 6242 - 6246
• Main Authors: Murata, Yoshihiko, Lightfoote, Paula M., Biear, Jamie N., Falsey, Ann R., Walsh, Edward E.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 31.08.2010; Elsevier; Elsevier Limited
• Subjects: Age; Aged; Aged, 80 and over; Allergy and Immunology; Antibodies, Viral - immunology; Applied microbiology; Attachment protein; Biological and medical sciences; Chronic obstructive pulmonary disease; Derivatives; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Glutathione transferase; Heart attacks; Hep G2 Cells; Hospitalization; Humans; Humoral response; Immunity, Humoral; Immunoglobulin G - immunology; Microbiology; Middle Aged; Plasmids; Polymerase chain reaction; Proteins; Respiratory syncytial virus; Respiratory Syncytial Virus Infections - immunology; Respiratory Syncytial Virus Infections - prevention & control; Respiratory Syncytial Virus Vaccines - immunology; Respiratory Syncytial Virus, Human - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viral Envelope Proteins - immunology; Attachment protein; Respiratory syncytial virus; Humoral response; Humoral immunity; Protein
• ISSN: 0264-410X; 1873-2518; 1873-2518
• DOI: 10.1016/j.vaccine.2010.07.011

To characterize the humoral response to the unglycosylated central region of the respiratory syncytial virus (RSV) attachment (G) protein, we generated glutathione S-transferase (GST)–RSV G subdomains (central core (CC), residues 151–190; proximal central core (PCC), 151–172; and distal central core (DCC), 173–190) to screen paired sera from RSV subtype A- or B-infected adults in hospitalized or outpatient settings. Following RSV infection, a ≥4-fold increase in homo- and heterosubtypic IgG response was noted in most subjects against the RSV G CC and PCC regions; in contrast, such titer increases against the RSV G DCC was only noted in a homosubtypic manner. Our results have implications for RSV G-based serological diagnostics and vaccine development.