Farnesol stimulates laccase production in Trametes versicolor

• Published in: Engineering in life sciences Vol. 16; no. 4; pp. 364 - 370
• Main Authors: Hu, Jianhua, Wang, Feng, Ma, Anzhou, Zhuang, Guoqiang, Liu, Ying, Lu, Jingsong, Guo, Chen, Liu, Chunzhao
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.05.2016; John Wiley & Sons, Inc; Wiley-VCH
• Subjects: Apoptosis; Aromatic amines; Biomass; Ethanol; Farnesol; Fermentation; Hydrogen peroxide; Hyperbranch; Laccase; Lignin; Manufacturers; Methylene blue; Mycelia; Oxidases; Quorum sensing; RhoA protein; Signal transduction; Trametes versicolor; Beijing China; United States > US; China
• ISSN: 1618-0240; 1618-2863
• DOI: 10.1002/elsc.201500082

Farnesol, a quorum‐sensing molecule, was used successfully to improve laccase production in submerged cultures of Trametes versicolor. At the optimal farnesol concentration of 60 μM added at the beginning of the culture, the extracellular laccase activity reached 629.3 U L−1 after 6 days of cultivation, which represented a 1.92‐fold increase relative to the control without farnesol treatment. The addition of farnesol resulted in an increase in the accumulation of H2O2 and an increased expression of the laccase (lac) gene and the RhoA gene. The RhoA gene correlated with hyperbranched mycelia, which facilitated the secretion of the intracellular laccase. This study provides a basis for understanding the induction mechanism of farnesol for enhancing laccase production.