Analysis of grain elements and identification of best genotypes for Fe and P in Afghan wheat landraces

• Published in: Breeding Science Vol. 66; no. 5; pp. 676 - 682
• Main Authors: Kondou, Youichi, Manickavelu, Alagu, Komatsu, Kenji, Arifi, Mujiburahman, Kawashima, Mika, Ishii, Takayoshi, Hattori, Tomohiro, Iwata, Hiroyoshi, Tsujimoto, Hisashi, Ban, Tomohiro, Matsui, Minami
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society of Breeding 2016; Japan Science and Technology Agency
• Subjects: best genotypes; Chemical composition; Cluster analysis; Crop improvement; elements; Genotypes; Germplasm; Grain; high-throughput method; Ions; Iron; Research Paper; Sample preparation; Sampling methods; Wheat; wheat landraces; wheat landraces; best genotypes; high-throughput method; elements
• ISSN: 1344-7610; 1347-3735
• DOI: 10.1270/jsbbs.16041

This study was carried out with the aim of developing the methodology to determine elemental composition in wheat and identify the best germplasm for further research. Orphan and genetically diverse Afghan wheat landraces were chosen and EDXRF was used to measure the content of some of the elements to establish elemental composition in grains of 266 landraces using 10 reference lines. Four elements, K, Mg, P, and Fe, were measured by standardizing sample preparation. The results of hierarchical cluster analysis using elemental composition data sets indicated that the Fe content has an opposite pattern to the other elements, especially that of K. By systematic analysis the best wheat germplasms for P content and Fe content were identified. In order to compare the sensitivity of EDXRF, the ICP method was also used and the similar results obtained confirmed the EDXRF methodology. The sampling method for measurement using EDXRF was optimized resulting in high-throughput profiling of elemental composition in wheat grains at low cost. Using this method, we have characterized the Afghan wheat landraces and isolated the best genotypes that have high-elemental content and have the potential to be used in crop improvement.