Iron Supplements Containing Lactobacillus plantarum 299v Increase Ferric Iron and Up-regulate the Ferric Reductase DCYTB in Human Caco-2/HT29 MTX Co-Cultures
Several human interventions have indicated that 299v ( 299v) increases intestinal iron absorption. The aim of the present study was to investigate possible effects of 299v on the mechanisms of iron absorption on the cellular level. We have previously shown that lactic fermentation of vegetables incr...
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Published in | Nutrients Vol. 10; no. 12; p. 1949 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
08.12.2018
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Several human interventions have indicated that
299v (
299v) increases intestinal iron absorption. The aim of the present study was to investigate possible effects of
299v on the mechanisms of iron absorption on the cellular level. We have previously shown that lactic fermentation of vegetables increased iron absorption in humans. It was revealed that the level of ferric iron [Fe (H₂O)₅]
was increased after fermentation. Therefore, we used voltammetry to measure the oxidation state of iron in simulated gastrointestinal digested oat and mango drinks and capsule meals containing
299v. We also exposed human intestinal co-cultures of enterocytes and goblet cells (Caco-2/HT29 MTX) to the supplements in order to study the effect on proteins possibly involved (MUC5AC, DCYTB, DMT1, and ferritin). We detected an increase in ferric iron in the digested meals and drinks containing
299v. In the intestinal cell model, we observed that the ferric reductase DCYTB increased in the presence of
299v, while the production of mucin (MUC5AC) decreased independently of
299v. In conclusion, the data suggest that the effect of
299v on iron metabolism is mediated through driving the Fe
/DCYTB axis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2072-6643 2072-6643 |
DOI: | 10.3390/nu10121949 |