Comparative evaluation of salivary biomarker levels in e-Cigarette smokers and conventional smokers

• Published in: Journal of pharmacy & bioallied science Vol. 13; no. 6; pp. 1642 - 1645
• Main Authors: Verma, Abhishek, Anand, Kumar, Bhargava, Manish, Kolluri, Amulya, Kumar, Madhuresh, Palve, Devendra
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01.11.2021; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Wolters Kluwer - Medknow; Wolters Kluwer Medknow Publications
• Subjects: Analysis; Body mass index; C-reactive protein; Cigarette smoking; Cytokines; e-cigarette; Electronic cigarettes; Enzyme-linked immunosorbent assay; Gingival index; IL-1β; Inflammation; inflammatory mediators; Interleukin 1; Interleukin 1 receptor antagonist; Interleukin 1 receptors; Interleukin 10; Interleukin 6; Interleukin 8; Oral fluids; Original; plaque scores; Saliva; salivary biomarkers; Smokers; Smoking; Statistical analysis; Tumor necrosis factor-TNF; Tumor necrosis factor-α; inflammatory mediators; gingival index; salivary biomarkers; plaque scores; E-cigarette; smoking
• ISSN: 0975-7406; 0976-4879; 0975-7406
• DOI: 10.4103/jpbs.jpbs_393_21

Background: The cigarette smoking and its effect on the inflammatory cytokine levels in the smoker's saliva depicted the influence of electronic cigarettes on oral cytokine levels in oral fluids are scarce in the literature. Objectives: The present trial was conducted to compare and determine the proinflammatory and anti-inflammatory cytokines in whole stimulated saliva samples of electronic cigarette smokers, conventional smokers, and participants with no smoke exposure. Materials and Methods: Sixty adult participants were divided into the following four groups of nonsmokers, current smokers, smokers smoking both conventional and e-cigarettes, and e-cigarette smokers. The saliva samples were assessed for Interleukins (IL-1B, 6, 8, 10, and IL-1RA), C-reactive protein (CRP), and tumor necrosis factor-alpha (TNF-α) using enzyme-linked immunosorbent assay. Plaque scores and Gingival Index, and body mass index were also calculated. Results: Statistically significant (P < 0.05) and remarkable relationship was seen in plaque scores and IL 1RA, 1 β, and 10 with the respective values as-0.285, 0.268, and 0.267. Regarding anti-inflammatory cytokines, CRP, IL-10, and IL-RA had the P-value of 0.073, 0.945, and 0.834 respectively. When these values were evaluated for proinflammatory cytokines, the P values were 0.0001, 0.019, 0.991, and 903 for TNF-α, IL-1 β, IL-6, and IL-8, respectively. These results were statistically significant for TNF-α (P = 0.001). Conclusion: Within its limitations, the present study concludes that smoking e-cigarettes whether solely or in combination with conventional smoking increases the levels of proinflammatory cytokines such as TNF-α and IL-1 β with decreased counter IL-1RA levels.