Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract

• Published in: Applied and Environmental Microbiology Vol. 75; no. 6; pp. 1534 - 1545
• Main Authors: Turroni, Francesca, Foroni, Elena, Pizzetti, Paola, Giubellini, Vanessa, Ribbera, Angela, Merusi, Paolo, Cagnasso, Patrizio, Bizzarri, Barbara, de'Angelis, Gian Luigi, Shanahan, Fergus, van Sinderen, Douwe, Ventura, Marco
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.2009; American Society for Microbiology (ASM)
• Subjects: adults; Age composition; Age Factors; Bacteria; Bacteriology; Bifidobacterium - classification; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Bifidobacterium adolescentis; Bifidobacterium animalis; Bifidobacterium bifidum; Bifidobacterium breve; Bifidobacterium dentium; Bifidobacterium longum; Bifidobacterium pseudocatenulatum; Bifidobacterium pseudolongum; Biological and medical sciences; Digestive system; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Ecological distribution; Feces; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Gastrointestinal Tract - microbiology; Genes; Genes, rRNA; Genetic Variation; Health promotion; human population; Humans; intestinal microorganisms; Intestinal Mucosa - microbiology; Microbial Ecology; Microbiology; Molecular Sequence Data; nucleotide sequences; Phylogeny; Ribonucleic acid; ribosomal RNA; RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; selective media; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Human; Lactic acid bacteria; Bifidobacterium; Actinobacteridae; Eubacteria; Diversity; Gut; Actinobacteria; Bacteria; Bifidobacteriaceae; Bifidobacteriales
• ISSN: 0099-2240; 1098-5336; 1098-5336; 1098-6596
• DOI: 10.1128/aem.02216-08

Although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. We performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rRNA gene sequences (16S rRNA gene and internally transcribed spacer [ITS] 16S-23S spacer sequences) of isolated colonies. A total of 900 isolates were collected, of which 704 were shown to belong to bifidobacteria. Analyses showed that the culturable bifidobacterial population from intestinal and fecal samples include six main phylogenetic taxa, i.e., Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bifidobacterium adolescentis, Bifidobacterium pseudolongum, Bifidobacterium breve, and Bifidobacterium bifidum, and two species mostly detected in fecal samples, i.e., Bifidobacterium dentium and Bifidobacterium animalis subp. lactis. Analysis of bifidobacterial distribution based on age of the subject revealed that certain identified bifidobacterial species were exclusively present in the adult human gut microbiota whereas others were found to be widely distributed. We encountered significant intersubject variability and composition differences between fecal and mucosa-adherent bifidobacterial communities. In contrast, a modest diversification of bifidobacterial populations was noticed between different intestinal regions within the same individual (intrasubject variability). Notably, a small number of bifidobacterial isolates were shown to display a wide ecological distribution, thus suggesting that they possess a broad colonization capacity.