mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines

• Published in: Data in brief Vol. 7; pp. 641 - 647
• Main Authors: Gómez-Sánchez, Rubén, Yakhine-Diop, Sokhna M.S., Rodríguez-Arribas, Mario, Bravo-San Pedro, José M., Martínez-Chacón, Guadalupe, Uribe-Carretero, Elisabet, Pinheiro de Castro, Diana C.J., Pizarro-Estrella, Elisa, Fuentes, José M., González-Polo, Rosa A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.06.2016; Elsevier
• Subjects: Autophagy; Biochemistry, Molecular Biology; Cellular Biology; Data; LC3; Life Sciences; p62; Western-blot; PBS; MEFs; SDS; TBST; Autophagy; p62; SB; HFs; FBS; qPCR; Baf. A1; EBSS; GAPDH; LC3; Western-blot; SDS, Sodium dodecyl sulfate; LC3, Microtubule-associated protein 1 light chain 3; EBSS, Earle׳s Balanced Salt Solution; TBST, Tris-buffered saline with Tween 20; HFs, Human fibroblasts; SB, Sample buffer; qPCR, Quantitative PCR; Baf. A1, Bafilomycin A1; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; MEFs, Mouse embryonic fibroblasts; FBS, Fetal bovine serum; PBS, Phosphate-buffered saline
• ISSN: 2352-3409; 2352-3409
• DOI: 10.1016/j.dib.2016.02.085

We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.