Influence of Citric Acid Amendments on the Availability of Weathered PCBs to Plant and Earthworm Species

• Published in: International journal of phytoremediation Vol. 8; no. 1; pp. 63 - 79
• Main Authors: White, Jason C., Parrish, Zakia D., Isleyen, Mehmet, Gent, Martin P. N., Iannucci-Berger, William, Eitzer, Brian D., Kelsey, Jason W., Mattina, Maryjane Incorvia
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis Group 01.01.2006; Taylor & Francis Ltd
• Subjects: Acids; Animals; Biodegradation, Environmental; Biological Availability; Citric acid; Citric Acid - pharmacology; Contaminants; Cucumis - metabolism; Cucumis sativus; Cucurbita - metabolism; Cucurbita pepo; Eisenia foetida; Lumbricus terrestris; Lupinus albus; Oligochaeta - metabolism; PCB; Pepo; phytoextraction; Plant Leaves - metabolism; Plant Roots - metabolism; Plant Stems - metabolism; Plants (organisms); Polychlorinated biphenyls; Polychlorinated Biphenyls - analysis; Polychlorinated Biphenyls - pharmacokinetics; POPs; Roots; Soil (material); Soil contaminants; Soil contamination; Soil Pollutants - analysis; Soil Pollutants - pharmacokinetics; Stems; Vegetables; Worms; Zucchini
• ISSN: 1522-6514; 1549-7879
• DOI: 10.1080/15226510500507102

A series of small and large pot trials were conducted to assess the phytoextraction potential of several plant species for weathered polychlorinated biphenyls (PCBs) in soil (105 μ g/g Arochlor 1268). In addition, the effect of citric acid on PCB bioavailability to both plants and earthworms was assessed. Under small pot conditions (one plant, 400 g soil), three cucurbits (Cucurbita pepo ssp pepo [zucchini] and ssp ovifera [nonzucchini summer squash], Cucumis sativus, cucumber) accumulated up to 270 μg PCB/g in the roots and 14 μg/g in the stems, resulting in 0.10% contaminant removal from soil. Periodic 1 mM subsurface amendments of citric acid increased the stem and leaf PCB concentration by 330 and 600%, respectively, and resulted in up to a 65% increase in the total amount of contaminant removed from soil. Although citric acid at 10 mM more than doubled the amount of PCB desorbed in abiotic batch slurries, contaminant accumulation by two earthworm species (Eisenia foetida and Lumbricus terrestris) was unaffected by citric acid at 1 and 10 mM and ranged from 11-15 μg/g. Two large pot trials were conducted in which cucurbits (C. pepo ssp pepo and ssp ovifera, C. sativus) and white lupin (Lupinus albus) were grown in 70 kg of PCB-contaminated soil. White lupin was the poorest accumulator of PCBs, with approximately 20 μ g/g in the roots and 1 μ g/g in the stems. Both C. pepo ssp ovifera (summer squash) and C. sativus (cucumber) accumulated approximately 65-100 μ g/g in the roots and 6-10 μ g/g in the stems. C. pepo ssp pepo (zucchini) accumulated significantly greater levels of PCB than all other species, with 430 μg/g in the roots and 22 μ g/g in the stems. The mechanism by which C. pepo spp pepo extracts and translocates weathered PCBs is unknown, but confirms earlier findings on the phytoextraction of other weathered persistent organic pollutants such as chlordane, p,p′-DDE, and polycyclic aromatic hydrocarbons.