Label-free identification and chemical characterisation of single extracellular vesicles and lipoproteins by synchronous Rayleigh and Raman scattering

Extracellular vesicles (EVs) present in blood originate from cells of different origins such as red blood cells (RBCs), platelets and leukocytes. In patients with cancer, a small portion of EVs originate from tumour cells and their load is associated with poor clinical outcome. Identification of the...

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Published inJournal of extracellular vesicles Vol. 9; no. 1; pp. 1730134 - n/a
Main Authors Enciso-Martinez, Agustin, Van Der Pol, Edwin, Hau, Chi M., Nieuwland, Rienk, Van Leeuwen, Ton G., Terstappen, Leon W.M.M., Otto, Cees
Format Journal Article
LanguageEnglish
Published Sweden Taylor & Francis 01.01.2020
John Wiley & Sons, Inc
Wiley
Subjects
Blood
Blood platelets
Erythrocytes
Extracellular vesicles
Flow cytometry
label-free
Lasers
Leukocytes
Light
Light scattering
Lipoproteins
Nanoparticles
optical trapping
Particle size
Penicillin
Plasma
Prostate cancer
Raman scattering
Rayleigh scattering
single particle
Spectrum analysis
Tumors
Netherlands
United Kingdom > UK
United States > US
Germany
single particle
Rayleigh scattering
label-free
Raman scattering
optical trapping
Extracellular vesicles
lipoproteins
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Summary:Extracellular vesicles (EVs) present in blood originate from cells of different origins such as red blood cells (RBCs), platelets and leukocytes. In patients with cancer, a small portion of EVs originate from tumour cells and their load is associated with poor clinical outcome. Identification of these tumour-derived extracellular vesicles (tdEVs) is difficult as they are outnumbered by EVs of different tissue of origin as well a large number of lipoproteins (LPs) that are in the same size range. In order to detect tdEVs from the abundant presence of other particles, single-particle techniques are necessary. Here, synchronous Rayleigh and Raman scattering is used for that purpose. This combination of light scattering techniques identifies optically trapped single particles based on Rayleigh scattering and distinguishes differences in chemical composition of particle populations based on Raman scattering. Here, we show that tdEVs can be distinguished from RBC EVs and LPs in a label-free manner and directly in suspension.
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ISSN:2001-3078
2001-3078
DOI:10.1080/20013078.2020.1730134