Pre-organized Guide RNA in the Cas9 Complex Is Ready for the Selection of Target Double-Stranded DNA

The 3 D structure of Cas9 bound with sgRNA was solved by X‐ray crystal‐structure analysis. The conformation change in SpyCas9 upon binding to sgRNA changes Cas9 to target‐DNA‐recognition mode in which seed segments in sgRNA are pre‐ordered in an A‐type conformation.

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Bibliographic Details
Published inChembiochem : a European journal of chemical biology Vol. 16; no. 16; pp. 2273 - 2275
Main Authors Kamiya, Yukiko, Asanuma, Hiroyuki
Format Journal Article
LanguageEnglish
Published Germany Blackwell Publishing Ltd 02.11.2015
Wiley Subscription Services, Inc
Subjects
CRISPR-Cas Systems - genetics
CRISPR/Cas
crystal structures
Crystallography, X-Ray
DNA - chemistry
DNA - metabolism
DNA silencing
Gene Silencing
genome editing
Nucleic Acid Conformation
Protein Structure, Tertiary
RNA, Guide, CRISPR-Cas Systems - chemistry
RNA, Guide, CRISPR-Cas Systems - metabolism
seed RNA
seed RNA
genome editing
DNA silencing
CRISPR/Cas
crystal structures
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Summary:The 3 D structure of Cas9 bound with sgRNA was solved by X‐ray crystal‐structure analysis. The conformation change in SpyCas9 upon binding to sgRNA changes Cas9 to target‐DNA‐recognition mode in which seed segments in sgRNA are pre‐ordered in an A‐type conformation.
Bibliography:istex:840FC4D243B8D8B6130B50D01C9E13CA81DF590D
ark:/67375/WNG-GS10Q8R8-4
ArticleID:CBIC201500424
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201500424