SDF-1／CXCR7对胃癌SGC-7901细胞合成及分泌炎症因子的影响

摘要：目的探讨SDF-1／CXCR7对胃癌SGC-7901细胞合成及分泌炎症因子的影响及其机制。方法采用CXCR7的shRNA慢病毒表达载体感染人胃癌SGC-7901细胞，Real-timePCR及Westernblot检测CXCR7mRNA和蛋白表达；应用SDF-1干预，分为4组：阴性对照组（LushRNA-NC），LV-shRNA-NC＋SDF-1组，CXCR7沉默组（LV-shRNA-CXCR7），LV—shRNA-CXCR7＋SDF-1组。采用Real-timePCR检测炎症因子TNF-a、IL-113、IL-6、IL-8的mRNA表达；采用ELISA检测细胞上清液TNF-a、IL-1...

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Bibliographic Details
Published in西安交通大学学报（医学版） Vol. 38; no. 1; pp. 34 - 38
Main Author 师阿盟 史海涛 董蕾 许绍娴 郭晓燕 王婷
Format Journal Article
LanguageChinese
Published 西安交通大学第二附属医院消化内科，陕西省胃肠动力疾病研究重点实验室，陕西省胃肠疾病临床研究中心，陕西西安 710004 2017
Subjects
CXC趋化因子受体7
NF-kB
基质细胞衍生因子-1（SDF-1）
炎症因子
胃癌
NF-κB
inflammatory cytokine
gastric cancer
炎症因子
SDF-1
胃癌
基质细胞衍生因子-1 (SDF-1 )
CXCR7
CXC趋化因子受体 7
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ISSN1671-8259
DOI10.7652/jdyxb201701007

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Summary:摘要：目的探讨SDF-1／CXCR7对胃癌SGC-7901细胞合成及分泌炎症因子的影响及其机制。方法采用CXCR7的shRNA慢病毒表达载体感染人胃癌SGC-7901细胞，Real-timePCR及Westernblot检测CXCR7mRNA和蛋白表达；应用SDF-1干预，分为4组：阴性对照组（LushRNA-NC），LV-shRNA-NC＋SDF-1组，CXCR7沉默组（LV-shRNA-CXCR7），LV—shRNA-CXCR7＋SDF-1组。采用Real-timePCR检测炎症因子TNF-a、IL-113、IL-6、IL-8的mRNA表达；采用ELISA检测细胞上清液TNF-a、IL-113、IL-6、IL-8的蛋白含量；采用Westernblot检测NF-kB通路的变化。结果①Real-timePCR及Westernblot结果提示，CXCR7沉默组sac-7901细胞CXCR7mRNA及蛋白的表达水平较阴性对照组显著降低（P均〈0．01），阴性对照组与空白对照组间无明显差异。②与LV-shRNA．Nc组相比，Lv-shRNA-NC＋SDF-1组IL-6及IL-8的mRNA表达及分泌明显增加（P〈0．01），LV-shRNA-CXCR7组IL-6及IL8mRNA表达及分泌减少（P〈0．05）；与LV—shRNA—NC＋SDF-1组相比，Lv-shRNA-CXCR7＋SDF-1组IL-6及IL-8mRNA表达及分泌明显减少（P〈0．01）。而TNF-Ⅸ及IL-18的mRNA表达及分泌在4组间无明显差异。③NF-eBp65核内表达、t-IkBa及p-IKBa表达在4组间均无明显差异。站论SDF-1可能通过CXCR7促进SGC-790l细胞合成及分泌炎症因子IL-6及IL-8，但不依赖于NF-KB通路。
Bibliography:Objective To investigate the effect of SDF-1/CXCR7 on inflammatory cytokine synthesis and secretion in gastric cancer SGC-7901 cells. Methods CXCR7 gene in SGC-7901 cells was silenced by shRNA lentiviral vector and the expression of CXCR7 was detected using Western blot and Real-time PCR. There were four groups as follows.. LV-shRNA-NC, LV-shRNA-NC＋ SDF-1, LV-shRNA-CXCRT, and LV-shRNA-CXCR7-F SDF-1 groups. Real-time PCR was used to detect the mRNA expressions of TNF-α, IL-1β, IL-6 and IL-8. ELISA was used to detect the protein levels of TNF-α, IL-1β, IL-6 and IL-8 in the culture supernatant. Western blot was used to detect the protein expressions of NF-kB pathway. Results （1） Transfection of SGC-7901 cells with CXCR7-shRNA lentiviral vector resulted in a significantly decreased expression of CXCR7 at both mRNA and protein levels （all P〈 0.01）. （3） Compared with those in LV-shRNA-NC group, IL-6 and IL-8 mRNA expressions and protein levels in the culture supernatant were increased in LV-shRNA-NC＋ SDF-1 group （P
ISSN:1671-8259
DOI:10.7652/jdyxb201701007