Visualizing Dynamic Performance of Lipid Droplets in a Parkinson’s Disease Model via a Smart Photostable Aggregation-Induced Emission Probe

• Published in: iScience Vol. 21; pp. 261 - 272
• Main Authors: Li, Lihua, Zhou, Fan, Gao, Qun, Lu, Yao, Xu, Xingyi, Hu, Rong, Wang, Zhiming, Peng, Mingying, Yang, Zhongmin, Tang, Ben Zhong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22.11.2019; Elsevier BV; Elsevier
• Subjects: Chemical Synthesis; Disease; Materials Design; Q; Science; Disease; Chemical Synthesis; Materials Design
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2019.10.027

Parkinson’s disease (PD) is a complex neurodegenerative disease affected by diverse factors, and lipid droplets (LDs) are increasingly recognized as major players in PD because of their relevance to neuron activity. However, long-term dynamic changes of LDs and their relative activity remain unclear. Here, an aggregation-induced emission (AIE) probe named 2-DPAN was prepared and employed to visualize dynamic processes of LDs in a 6-hydroxydopamine model of PD for the first time, and LDs' accumulation-peak/plateau-decrease were confirmed. We further found a close relationship between LDs and variation in mitochondrial activity. Strikingly, the progression of cell death was accelerated by lipase, whereas pre-stimulation of LDs by unsaturated fatty acid-oleic acid decreased the death process by inhibiting excessive reactive oxygen species (ROS) and fatty acid production, thereby protecting mitochondria. The utilization of 2-DPAN demonstrates the importance of LDs in neuronal homeostasis, and effective tuning of LDs may prevent or inhibit PD progression. [Display omitted] •2-DPAN monitors the dynamic changes of Lipid droplets (LDs) in Parkinson disease•LDs' dynamic change process including three phases, accumulation-plateau-decrease•LDs' change trend was highly correlated with mitochondrial disruption•Efficient tuning of LDs could slow the PD progress Disease; Chemical Synthesis; Materials Design