Tobacco mosaic virus Induced Alterations in the Gene Expression Profile of Arabidopsis thaliana

• Published in: Molecular plant-microbe interactions Vol. 16; no. 8; pp. 681 - 688
• Main Authors: Golem, Sheetal, Culver, James N
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.08.2003; American Phytopathological Society; The American Phytopathological Society
• Subjects: antioxidants; Arabidopsis - genetics; Arabidopsis - virology; Arabidopsis thaliana; Biological and medical sciences; complementary DNA; ecotypes; enzymes; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant; genes; growth retardation; Immunity, Innate - genetics; leaf curling; leaves; messenger RNA; microarray technology; necrosis; Oligonucleotide Array Sequence Analysis - methods; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - genetics; Plant Diseases - virology; Plant viruses and viroids; Signal Transduction - genetics; Systematics. Structure, properties and multiplication. Genetics; Tobacco mosaic virus; Tobacco Mosaic Virus - growth & development; transcription factors; transcriptome; transporters; virus-host interactions; viruses; Disease development; Plant pathogen; Tobamovirus; Gene expression; Host virus relation; Virus; Arabidopsis thaliana; Cruciferae; Tobacco mosaic virus; Dicotyledones; Angiospermae; Spermatophyta; Experimental plant
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi.2003.16.8.681

In this study, mRNA profiles generated from cDNA microarrays were used to identify gene expression changes in Arabidopsis thaliana ecotype Shahdara infected with Tobacco mosaic virus (TMV). Shahdara is a susceptible TMV host, permitting rapid accumulations of virus in both inoculated and systemic tissues, accompanied by defined disease symptoms that include stunting, necrosis, and leaf curling. Gene expression profiles were monitored in whole tissues of inoculated leaves at four days postinoculation (dpi) and in systemically infected leaves at 14 dpi. Microarrays contained cDNAs representing between 8,000 and 10,000 Arabidopsis genes. Expression analysis identified 68 genes that displayed significant and consistent changes in expression levels, either up or down, in either TMV inoculated or systemically infected tissues, or both. Identified TMV-responsive genes encode a diverse array of functional proteins that include transcription factors, antioxidants, metabolic enzymes, and transporters. Thus, the TMV infection process has a significant impact on a wide array of cellular processes that likely reflect the biochemical and physiological changes involved in the development of this disease syndrome.