Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 105; no. 6; pp. 1955 - 1959
• Main Authors: High, Frances A, Lu, Min Min, Pear, Warren S, Loomes, Kathleen M, Kaestner, Klaus H, Epstein, Jonathan A
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 12.02.2008; National Acad Sciences
• Subjects: Animals; Aorta; Biological Sciences; Blood vessels; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Cardiovascular disease; Cells; Developmental biology; Embryology; Embryos; Endothelial cells; Endothelium; Endothelium, Vascular - metabolism; Gene expression; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Jagged-1 Protein; Ligands; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; Mice, Knockout; Molecules; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - metabolism; Mutation; Phenotypes; Protein Binding; Receptors, Notch - metabolism; Serrate-Jagged Proteins; Smooth muscle; Yolk sac
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0709663105

The Notch ligand Jagged1 (Jag1) is essential for vascular remodeling and has been linked to congenital heart disease in humans, but its precise role in various cell types of the cardiovascular system has not been extensively investigated. We show that endothelial-specific deletion of Jag1 results in embryonic lethality and cardiovascular defects, recapitulating the Jag1 null phenotype. These embryos show striking deficits in vascular smooth muscle, whereas endothelial Notch activation and arterial-venous differentiation appear normal. Endothelial Jag1 mutant embryos are phenotypically distinct from embryos in which Notch signaling is inhibited in endothelium. Together, these results imply that the primary role of endothelial Jag1 is to potentiate the development of neighboring vascular smooth muscle.