Intraarticular injection of processed lipoaspirate cells has anti-inflammatory and analgesic effects but does not improve degenerative changes in murine monoiodoacetate-induced osteoarthritis

• Published in: BMC musculoskeletal disorders Vol. 20; no. 1; p. 335
• Main Authors: Sakamoto, Takumi, Miyazaki, Tsuyoshi, Watanabe, Shuji, Takahashi, Ai, Honjoh, Kazuya, Nakajima, Hideaki, Oki, Hisashi, Kokubo, Yasuo, Matsumine, Akihiko
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.07.2019; BioMed Central; BMC
• Subjects: Adipose derived-mesenchymal stem cells (AD-MSCs); Adipose tissue; Adipose Tissue - cytology; Animals; Anti-inflammatory agents; Arthralgia - diagnosis; Arthralgia - etiology; Arthralgia - therapy; Arthritis, Experimental - chemically induced; Arthritis, Experimental - therapy; Biomarkers - analysis; Calcitonin; Cartilage, Articular - pathology; Clinical trials; Complications and side effects; Elderly; Female; Ganglia, Spinal - pathology; Ganglion cysts; Genes; Health aspects; Humans; Inflammation; Injections, Intra-Articular; Intra-articular injection; Iodoacetic Acid - toxicity; Knee Joint - innervation; Knee Joint - pathology; Medical research; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal stem cells (MSCs); Monoiodoacetate (MIA); Neurons; Osteoarthritis; Osteoarthritis (OA); Osteoarthritis - chemically induced; Osteoarthritis - pathology; Osteoarthritis - therapy; Pain Measurement - methods; Peptides; Processed lipoaspirate (PLA) cells; Rats; Rats, Sprague-Dawley; Risk factors; Stem cell research; Stem cell transplantation; Stem cells; Treatment Outcome; Monoiodoacetate (MIA); Intra-articular injection; Sprague-Dawley rats; Calcitonin gene-related peptide (CGRP); Osteoarthritis (OA); Mesenchymal stem cells (MSCs); Adipose derived-mesenchymal stem cells (AD-MSCs); Dorsal root ganglion (DRG); Processed lipoaspirate (PLA) cells; Substance P (SP)
• ISSN: 1471-2474; 1471-2474
• DOI: 10.1186/s12891-019-2710-1

Previous basic research and clinical studies examined the effects of mesenchymal stem cells (MSCs) on regeneration and maintenance of articular cartilage. However, our pilot study suggested that MSCs are more effective at suppressing inflammation and pain rather than promoting cartilage regeneration in osteoarthritis. Adipose tissue is considered a useful source of MSCs; it can be harvested easily in larger quantities compared with the bone marrow. The present study was designed to evaluate the anti-inflammatory, analgesic, and regenerative effects of intra-articularly injected processed lipoaspirate (PLA) cells (containing adipose-derived MSCs) on degenerative cartilage in a rat osteoarthritis model. PLA cells were isolated from subcutaneous adipose tissue of 12-week-old female Sprague-Dawley rats. Osteoarthritis was induced by injection of monoiodoacetate (MIA). Each rat received 1 × 10 MSCs into the joint at day 7 (early injection group) and day 14 (late injection group) post-MIA injection. At 7, 14, 21 days after MIA administration, pain was assessed by immunostaining and western blotting of dorsal root ganglion (DRG). Cartilage quality was assessed macroscopically and by safranin-O and H&E staining, and joint inflammation was assessed by western blotting of the synovium. The early injection group showed less cartilage degradation, whereas the late injection group showed cartilage damage similar to untreated OA group. The relative expression level of CGRP protein in DRG neurons was significantly lower in the two treatment groups, compared with the untreated group. Intra-articular injection of PLA cells prevented degenerative changes in the early injection group, but had little effect in promoting cartilage repair in the late injection group. Interestingly, intra-articular injection of PLA cells resulted in suppression of inflammation and pain in both OA groups. Further studies are needed to determine the long-term effects of intra-articular injection of PLA cells in osteoarthritis.