Mitogenicity and Adjuvanticity of Lipopolysaccharide from a Marine Bacterium, Vibrio anguillarum, in Mice

• Published in: Chemical & pharmaceutical bulletin Vol. 32; no. 10; pp. 4165 - 4169
• Main Authors: SHIMIZU, TADAYORI, MATSUSAKA, EIICHIRO, MASUZAWA, TOSHIYUKI, YANAGIHARA, YASUTAKE, MIFUCHI, ICHIJI, IGUCHI, TAKEHIRO, KONDO, SEIICHI, HISATSUNE, KAZUHITO
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.01.1984; Maruzen
• Subjects: adjuvanticity; Adjuvants, Immunologic; Animals; Biological and medical sciences; Female; General pharmacology; Male; Marine; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mitogens; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Polysaccharides, Bacterial - pharmacology; Vibrio - immunology; Vibrio anguillarum; Marine environment; Lipopolysaccharide; Bacteria; Immunostimulant agent; Mitogen; Structure activity relation
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.32.4165

The effects of lipopolysaccharides (LPS) extracted from three O-serotype (A, B, and C) strains of Vibrio anguillarum on the murine immune response were studied. Although the LPSs lack 2-keto-3-deoxyoctonate (KDO) and the sugar compositions of their core regions are markedly different, depending on the O-serotype, they were all able to induce a mitogenic effect on in vitro-cultured spleen cells of C57BL/6 mice. The LPS was capable of increasing the incorporation of 3H-thymidine into spleen cells that had been treated with rabbit antithymocyte serum in the presence of complement in order to kill T-lymphocytes. When sheep erythrocytes and LPS were injected intraperitoneally into BALB/c mice, the LPSs exhibited an enhancing effect on the antibody response regardless of the serotype. These findings suggest that these biological effects of V. anguillarum LPSs from different O-serotype strains are little affected by the absence of KDO and the differences in sugar composition.