Soluble matrix from osteoblastic cells induces mineralization by dental pulp cells

Dental pulp cells have a capacity to differentiate into mineralization-inducing cells. To clarify the molecular mechanism, we established an in vitro mineralization-inducing system by rat clonal dental pulp cell line, RPC-C2A, and tried to purify a mineralization-inducing factor in conditioned mediu...

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Published inThe Journal of Medical Investigation Vol. 53; no. 3,4; pp. 297 - 302
Main Authors Abe, Kaori, Yamashita, Kikuji, Ruspita, Intan, Miyoshi, Keiko, Horiguchi, Taigo, Ueno, Akemichi, Noma, Takafumi
Format Journal Article
LanguageEnglish
Published Japan The University of Tokushima Faculty of Medicine 01.08.2006
Subjects
Animals
Calcification, Physiologic - drug effects
Calcification, Physiologic - physiology
Cell Line
Collagen Type I - metabolism
Collagen Type I - pharmacology
Dental Pulp - cytology
Dental Pulp - metabolism
Dental Pulp - physiology
dental pulp cells
extracellular matrix
Extracellular Matrix - physiology
Extracellular Matrix Proteins - metabolism
Extracellular Matrix Proteins - pharmacology
mineralization
osteoblast
Osteoblasts - cytology
Osteoblasts - metabolism
Osteoblasts - physiology
Osteogenesis - drug effects
Osteogenesis - physiology
Rats
Skull - cytology
Skull - metabolism
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ISSN1343-1420
1349-6867
DOI10.2152/jmi.53.297

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Summary:Dental pulp cells have a capacity to differentiate into mineralization-inducing cells. To clarify the molecular mechanism, we established an in vitro mineralization-inducing system by rat clonal dental pulp cell line, RPC-C2A, and tried to purify a mineralization-inducing factor in conditioned medium (CM) from preosteoblastic MC3T3-E1 cells. The active factor was impermeable to an ultrafiltrating membrane, and sedimented by ultracentrifugation. The sedimented factor was found as a needle-like structure about 1.3 µm in average length as observed by transmission electron microscopy. The factor contained type I collagen, suggesting not a matrix vesicle, but a soluble matrix. The mineralization-inducing activity was also detected in CM from primary culture of rat calvaria (RC) cells. These results suggested that the soluble matrices from osteoblastic cells serve, at least in part, as differentiation-inducing agents. J. Med. Invest. 53: 297-302, August, 2006
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ISSN:1343-1420
1349-6867
DOI:10.2152/jmi.53.297