Four-dimensional motility tracking of biological cells by digital holographic microscopy

Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the informatio...

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Bibliographic Details
Published inJournal of biomedical optics Vol. 19; no. 4; p. 045001
Main Authors Yu, Xiao, Hong, Jisoo, Liu, Changgeng, Cross, Michael, Haynie, Donald T, Kim, Myung K
Format Journal Article
LanguageEnglish
Published United States Society of Photo-Optical Instrumentation Engineers 01.04.2014
Subjects
Cell Movement - physiology
Cell Tracking - methods
Cryptophyta - cytology
Digital
Holograms
Holography
Holography - methods
Microfibers
Micrometers
Microscopy
Models, Biological
Research Papers: General
Three dimensional
Tracking
three-dimensional profiling
cells
four-dimensional tracking
microspheres
microscopy
digital holography
microfibers
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Summary:Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the information about their three-dimensional structures. Here, we have utilized DHM to visualize suspended microspheres and microfibers in three dimensions, and record the four-dimensional trajectories of free-swimming cells in the absence of mechanical focus adjustment. The displacement of microfibers due to interactions with cells in three spatial dimensions has been measured as a function of time at subsecond and micrometer levels in a direct and straightforward manner. It has thus been shown that DHM is a highly efficient and versatile means for quantitative tracking and analysis of cell motility.
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ISSN:1083-3668
1560-2281
1560-2281
DOI:10.1117/1.JBO.19.4.045001