A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing

• Published in: Analytical and bioanalytical chemistry Vol. 407; no. 14; pp. 4005 - 4013
• Main Authors: Ewald, Melanie, Fechner, Peter, Gauglitz, Günter
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2015; Springer; Springer Nature B.V
• Subjects: Analysis; Analytical Chemistry; Animals; Antibodies; Antibodies, Bacterial; Bacterial infections; Biochemistry; Biological markers; Biomarkers; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; C-reactive protein; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chickens; Direct Optical Detection; Food Science; Glass substrates; Infections; Laboratory Medicine; Monitoring/Environmental Analysis; Pathogens; Point-of-Care Systems; Poultry Diseases - diagnosis; Poultry Diseases - microbiology; Research Paper; Salmonella; Salmonella - isolation & purification; Salmonella Infections, Animal - diagnosis; Salmonella Infections, Animal - microbiology; Temperature effects; Germany; CRP; infection; Multi-analyte; Optical biosensors; Point-of-need (PON); Label-free; 1-lambda-Reflectometry
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-015-8562-0

For the first time, a multi-analyte biosensor platform has been developed using the label-free 1-lambda-reflectometry technique. This platform is the first, which does not use imaging techniques, but is able to perform multi-analyte measurements. It is designed to be portable and cost-effective and therefore allows for point-of-need testing or on-site field-testing with possible applications in diagnostics. This work highlights the application possibilities of this platform in the field of animal testing, but is also relevant and transferable to human diagnostics. The performance of the platform has been evaluated using relevant reference systems like biomarker (C-reactive protein) and serology (anti- Salmonella antibodies) as well as a panel of real samples (animal sera). The comparison of the working range and limit of detection shows no loss of performance transferring the separate assays to the multi-analyte setup. Moreover, the new multi-analyte platform allows for discrimination between sera of animals infected with different Salmonella subtypes.