Biocompatibility enhancement of graphene oxide-silver nanocomposite by functionalisation with polyvinylpyrrolidone

• Published in: IET nanobiotechnology Vol. 13; no. 8; pp. 816 - 823
• Main Authors: Khalil, Wafaa A, Sherif, Hadeer H.A, Hemdan, Bahaa A, Khalil, Safaa K.H, Hotaby, Walid El
• Format: Journal Article
• Language: English
• Published: United States The Institution of Engineering and Technology 01.10.2019; Wiley
• Subjects: Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antibacterial activity; antimicrobial activity; biocompatibility; biomedical materials; BJ1 normal skin fibroblasts; Candida albicans; cell viability; Cells, Cultured; cellular biophysics; Coated Materials, Biocompatible - chemical synthesis; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Coated Materials, Biocompatible - standards; CO‐Ag; cytotoxicity; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - physiology; filled polymers; Fourier transform infrared spectra; graphene compounds; graphene oxide‐silver nanocomposite; Graphite - chemistry; Graphite - pharmacology; high‐resolution transmission electron microscopy; Humans; Materials Testing; Metal Nanoparticles - chemistry; Metal Nanoparticles - standards; Microbial Sensitivity Tests; microorganisms; morphological characterisations; nanocomposites; Nanocomposites - chemistry; Nanocomposites - standards; nanofabrication; nanomedicine; nanoparticles; polyvinylpyrrolidone; Povidone - chemistry; Povidone - pharmacology; Pseudomonas aeruginosa; Quality Improvement; Raman spectra; Research Article; silver; Silver - chemistry; Silver - pharmacology; Skin - cytology; Skin - drug effects; Staphylococcus aureus; structural characterisations; toxic materials; toxicology; transmission electron microscopy; ultraviolet spectra; UV‐visible spectra; visible spectra; X‐ray diffraction; antibacterial activity; Candida albicans; Raman spectra; UV-visible spectra; cellular biophysics; Fourier transform infrared spectra; nanofabrication; X-ray diffraction; nanocomposites; structural characterisations; visible spectra; nanoparticles; CO-Ag; morphological characterisations; toxic materials; Pseudomonas aeruginosa; transmission electron microscopy; BJ1 normal skin fibroblasts; Staphylococcus aureus; cytotoxicity; graphene compounds; toxicology; biocompatibility; polyvinylpyrrolidone; nanomedicine; antimicrobial activity; graphene oxide-silver nanocomposite; high-resolution transmission electron microscopy; ultraviolet spectra; cell viability; microorganisms; biomedical materials; filled polymers; silver
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2018.5321

Several materials such as silver are used to enhance graphene oxide (GO) sheets antimicrobial activity. However, these toxic materials decrease its biocompatibility and hinder its usage in many biological applications. Therefore, there is an urgent need to develop nanocomposites that can preserve both the antimicrobial activity and biocompatibility simultaneously. This work highlights the importance of functionalisation of GO sheets using Polyvinylpyrrolidone (PVP) and decorating them with silver nanoparticles (AgNPs) in order to enhance their antimicrobial activity and biocompatibility at the same time. The structural and morphological characterisations were performed by UV-Visible, Fourier transform infrared (FTIR), and Raman spectroscopic techniques, X-ray diffraction (XRD), and high-resolution transmission electron microscopy (HR-TEM). The antimicrobial activities of the prepared samples against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans were studied. The cytotoxicity of prepared materials was tested against BJ1 normal skin fibroblasts. The results indicated that the decoration with AgNPs showed a significant increase in the antimicrobial activity of GO and FGO sheets, and functionalisation of GO sheets and GO-Ag nanocomposite with PVP improved the cell viability about 40 and 35%, respectively.